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	Provedor de dados Electron. J. Biotechnol. (2) Autor Montoya Castaño,Dolly (2) Aristizábal Gutierrez,Fabio Ancizar (1) Bernal Morales,Jose Mauricio (1) Grosso,María Victoria (1) Moreno Solano,Nubia Carmenza (1) Quilaguy Ayure,Diana Milena (1) Revelo Romo,Dolly Margot (1) Suárez Moreno,Zulma Rocío (1) Mais... Palavra-chave 16S rDNA (1) Clostridium (1) Colony PCR (1) Degenerate primers (1) Genome size (1) PFGE (1) PHA synthase (1) PSOL1 (1) Polyhydroxyalcanoate (1) Seminested PCR (1) Mais... Tipo do documento Journal article (2) Ano 2007 (1) 2006 (1) País Chile (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Genome analysis of thirteen Colombian clostridial strains by pulsed field gel electrophoresis Electron. J. Biotechnol. Quilaguy Ayure,Diana Milena; Suárez Moreno,Zulma Rocío; Aristizábal Gutierrez,Fabio Ancizar; Bernal Morales,Jose Mauricio; Montoya Castaño,Dolly. Pulsed field gel electrophoresis was used for estimating the size of the genome and evaluating the presence of megaplasmids in 13 native Colombian solventogenic Clostridium strains. DNA preparation and purification were optimised for obtaining differentiated restriction fragments in electrophoresis. Genomic DNA was digested with ApaI, Eco52I, SmaI and XhoI enzymes. Estimated genome size for native strains ranged from 4.0 to 4.2 mega base pairs. Larger sized plasmids were detected and the presence of genes related to megaplasmid pSOL1 was determined by polymerase chain reaction. adc gene region amplification suggested that genes related to solventogenesis in native strains may be located in an extra-chromosomal element. Determining genome size provides... Tipo: Journal article Palavras-chave: Clostridium; Genome size; PFGE; PSOL1. Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500009 A most effective method for selecting a broad range of short and medium-chain-length polyhidroxyalcanoate producing microorganisms Electron. J. Biotechnol. Revelo Romo,Dolly Margot; Grosso,María Victoria; Moreno Solano,Nubia Carmenza; Montoya Castaño,Dolly. A molecular approach was used for selecting polyhydroxyalcanoate (PHA)-accumulating potential Gram-negative bacteria from different genera by colony polymerase chain reaction (PCR). Three degenerate primers were designed for amplifying a fragment from PHA synthase gene (phaC) (Class I), phaC1 and phaC2 (Class II) genes for detecting PHA-producing bacteria. Thirty-four out of 55 bacterial strains from the old collection selected using Sudan black B staining were phaC+. PCR was used for directly selecting 35 new collection bacterial strains; these strains were phaC+ and their ability to produce PHA was confirmed by Sudan black B staining. Four specific primers were designed on genes of Class II PHA biosynthesis operon. These primers were used for evaluating... Tipo: Journal article Palavras-chave: 16S rDNA; Colony PCR; Degenerate primers; PHA synthase; Polyhydroxyalcanoate; Seminested PCR. Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000300002 Registros recuperados: 2 Primeira ... 1 ... Última

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