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| Araya,Carolina; Peña,Elizabeth; Salazar,Erika; Román,Lisset; Medina,Claudia; Mora,Roxana; Aljaro,Agustín; Rosales,Inés-Marlene. |
| Big-vein disease (BVD) is a widespread and economically damaging disease in lettuce (Lactuca sativa L.). Typical symptoms are chlorotic clearing around leaf veins, leaf deformations, and impaired head development. In this research, we studied the relationship between symptom intensity and protein and viral RNA accumulation in infected plants. Naturally infected lettuce plants, from the field or greenhouse, were classified according to their symptomatology: mild, moderate, severe, and symptomless. Coat protein accumulation was evaluated by a double antibody sandwich/enzyme-linked immunosorbent assay (DAS-ELISA), and RNA levels were studied by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and quantitative RT-PCR. Virus coat... |
| Tipo: Journal article |
Palavras-chave: Lettuce big-vein disease; Symptom expression. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392011000100008 |
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| Mujica,María Valentina; Mora,Roxana; Rosales,Marlene; Sandoval,Claudio. |
| A vineyard in south-central Chile was surveyed during five growing seasons to analyze the increase over time in the number of plants infected with Grapevine leafroll-associated virus-1, -2 and -3. At the end of the study, GLRaV-3 was found to be the most prevalent virus, with 46% of plants infected by this pathogen. GLRaV-2 infected 13% of plants, and GLRaV-1 infected 12%. Considering all three viruses together, the prevalence of infection increased exponentially from 14% of the plants at the beginning of the study period to 68% at the end. The patterns of the spread of GLRaV-2 and GLRaV-3 were mostly along the rows, whereas the pattern of spread for GLRaV-1 could not be determined. |
| Tipo: Journal article |
Palavras-chave: Field transmission; Grapevine leafroll-associated virus; GLRaV-1; GLRaV-2; GLRaV-3; Virus diagnosis. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202013000100012 |
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