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| Registros recuperados: 10 | |
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| Fleury, Elodie; Huvet, Arnaud; Lelong, Christophe; De Lorgeril, Julien; Boulo, Viviane; Gueguen, Yannick; Bachere, Evelyne; Tanguy, Arnaud; Moraga, Dario; Fabioux, Caroline; Lindeque, Penelope; Shaw, Jenny; Reinhardt, Richard; Prunet, Patrick; Davey, Grace; Lapegue, Sylvie; Sauvage, Christopher; Corporeau, Charlotte; Moal, Jeanne; Gavory, Frederick; Wincker, Patrick; Moreews, Francois; Klopp, Christophe; Mathieu, Michel; Boudry, Pierre; Favrel, Pascal. |
| Background: Although bivalves are among the most-studied marine organisms because of their ecological role and economic importance, very little information is available on the genome sequences of oyster species. This report documents three large-scale cDNA sequencing projects for the Pacific oyster Crassostrea gigas initiated to provide a large number of expressed sequence tags that were subsequently compiled in a publicly accessible database. This resource allowed for the identification of a large number of transcripts and provides valuable information for ongoing investigations of tissue-specific and stimulus-dependant gene expression patterns. These data are crucial for constructing comprehensive DNA microarrays, identifying single nucleotide... |
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| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6770.pdf |
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| Genard, Bertrand; Miner, Philippe; Nicolas, Jean-louis; Moraga, Dario; Boudry, Pierre; Pernet, Fabrice; Tremblay, Rejean. |
| Background: Bacterial infections are common in bivalve larvae and can lead to significant mortality, notably in hatcheries. Numerous studies have identified the pathogenic bacteria involved in such mortalities, but physiological changes associated with pathogen exposure at larval stage are still poorly understood. In the present study, we used an integrative approach including physiological, enzymatic, biochemical, and molecular analyses to investigate changes in energy metabolism, lipid remodelling, cellular stress, and immune status of Crassostrea gigas larvae subjected to experimental infection with the pathogenic bacteria Vibrio coralliilyticus. Findings: Our results showed that V. coralliilyticus exposure induced (1) limited but significant increase... |
| Tipo: Text |
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| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00144/25504/23662.pdf |
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| Lacroix, Camille; Richard, Gaelle; Seguineau, Catherine; Guyomarch, Julien; Moraga, Dario; Auffret, Michel. |
| Brest harbor (Bay of Brest, Brittany, France) has a severe past of anthropogenic chemical contamination, but inputs tended to decrease, indicating a reassessment of its ecotoxicological status should be carried out. Here, native and caged mussels (Mytilus spp.) were used in combination to evaluate biological effects of chronic chemical contamination in Brest harbor. Polycyclic aromatic hydrocarbon (PAH) contamination was measured in mussel tissues as a proxy of harbor and urban pollution. Biochemical biomarkers of xenobiotic biotransformation, antioxidant defenses, generation of reducing equivalents, energy metabolism and oxidative damage were studied in both gills and digestive glands of native and caged mussels. In particular, activities of... |
| Tipo: Text |
Palavras-chave: Marine biomonitoring; Chronic pollution; Blue mussel Mytilus spp.; Caging; Oxidative stress; Energetic metabolism; Bay of Brest. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00255/36654/35795.pdf |
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| Boutet, Isabelle; Tanguy, Aurelie; Moraga, Dario. |
| Marine bivalve metabolism can be perturbed by hydrocarbon and pesticide pollution in coastal ecosystems. In this study, in the Pacific oyster, Crassostrea gigas, full-length cDNAs encoding two non-P450 phase I enzymes, flavin-containing monooxygenase 2 (FMO-2) and monamine oxidase A (MAO A), were characterized. Both sequences contained the co-factor fixation motifs characteristic of their respective enzyme families. Using reverse transcription polymerase chain reaction (RT-PCR), the messenger RNA (mRNA) transcription levels of these two enzymes in tissues of oysters exposed, under experimental conditions, to hydrocarbons and two pesticide treatments were investigated. The pesticide treatments were exposure to either glyphosate or to a mixture composed of... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; Flavin containing monooxygenase 2; Monoamine oxidase A. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-676.pdf |
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| Genard, Bertrand; Moraga, Dario; Pernet, Fabrice; David, Elise; Boudry, Pierre; Tremblay, Rejean. |
| Quantification of mRNA of genes related to metabolism, immunity and cellular stress was examined in relation to a massive mortality event during the culture of American oyster larvae, Crassostrea virginica which was probably, in regard to previous microbiological analysis, induced by vibrio infection. To document molecular changes associated with the mortality event, mRNA levels were compared to biochemical and physiological data, previously described in a companion paper. Among the 18 genes studied, comparatively to the antibiotic control, 10 showed a lower relative gene expression when the massive mortality occurred. Six of them are presumed to be related to metabolism, corroborating the metabolic depression associated with the mortality event suggested... |
| Tipo: Text |
Palavras-chave: Massive mortality; Relative gene expression; Metabolism; Immunity; Cellular stress; Larvae. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00075/18585/16138.pdf |
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| Tanguy, Arnaud; Boutet, Isabelle; Boudry, Pierre; Degremont, Lionel; Laroche, Jean; Moraga, Dario. |
| Phosphoglucomutase is a key enzyme in glycolysis and has been widely studied in vertebrates and some invertebrates but no molecular information is available in marine invertebrates despite the importance of this marker in ecological and genetical studies. In this work, we isolated a cDNA and the corresponding genomic sequence that encode PGM-2 locus in the Pacific oyster Crassostrea gigas. We used sequences drawn from the database to construct an evolutionary framework for examining the position of mollusc PGM sequences among prokaryotic and eukaryotic homologues and showed that oyster PGM gene organization was closer to vertebrates PGM genes than other invertebrates as previously found in other Lophotrochozoa species. We also investigated PGM mRNA... |
| Tipo: Text |
Palavras-chave: Gene expression; Xenobiotics; Mollusc; Phosphoglucomutase. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-2220.pdf |
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| Genard, Bertrand; Pernet, Fabrice; Lemarchand, Karine; Boudry, Pierre; Moraga, Dario; Tremblay, Rejean. |
| In this paper, biochemical and physiological analyses were used to characterize changes associated with mortality event occurred during veliger development of American oyster, Crassostrea virginica. Biochemical analyses included the evaluation of lipid classes, fatty acid composition and total protein content. Larval physiology was evaluated by studying feeding activity, enzymes related to energy metabolism, oxidative stress levels and enzymatic antioxidant defenses. These analyses were complemented by bacterial community analyses as well as by measuring larval oyster performance. We observed that mortality events coincided with (1) strong changes in the surrounding bacterial community; (2) a progressive decrease in feeding activity; (3) higher levels of... |
| Tipo: Text |
Palavras-chave: Massive mortality; Larvae; Microbial environment; Lipid; Fatty acids; Energy metabolism; Oxidative stress; Antioxidant enzymes; Crassostrea virginica. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00051/16231/13753.pdf |
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| Registros recuperados: 10 | |
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