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	Provedor de dados Genet. Mol. Biol. (2) Rev. Microbiol. (1) Autor Morais Jr.,Marcos Antonio de (3) Cavalcanti,Andre Ricardo de Oliveira (1) Ferreira,Luiz Carlos de Souza (1) Ferreira,Rita de Cássia Café (1) Guimarães,Katia Silva (1) Lima Filho,José Luiz de (1) Maia,Maria de Mascena Diniz (1) Melo,Eduardo Henrique Magalhães (1) Morais,Marcia Maria Camargo de (1) Silva,Walkiria Luckwu de Santana (1) Mais... Palavra-chave DNA binding site (1) DNA repair (1) Enzyme kinetics (1) Ester hydrolysis (1) Extracellular lipase (1) Fusarium solani (1) Gene promoter (1) Gene regulation (1) Transfac (1) Weight matrix (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Ano 2005 (1) 1999 (1) 1998 (1) País Brazil (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Mutagenic activation of CL64,855, an anti-Trypanosoma cruzi nitroderivant, by bacterial nitroreductases Genet. Mol. Biol. Morais Jr.,Marcos Antonio de; Ferreira,Rita de Cássia Café; Ferreira,Luiz Carlos de Souza. CL64,855 is a nitroimidazole-thiodiazole derivate with high anti-Trypanosoma cruzi activity. CL64,855-induced mutagenesis in the Salmonella/microsome test was detected by TA98 and TA98dnp6 strains, but not by the nitroreductase I-deficient TA98nr strain. The lack of mutagenic response of TA98nr was connected with its extreme resistance to the killing effect of the drug. Presence of S9 mix did not restore mutagenic activity of CL64,855 to the TA98nr strain. Additionally, CL64,855 was reduced in vitro by the nitroreductase I-proficient TA98 strain, mainly in the presence of oxygen, but not by the TA98nr strain. Mutagenic activity was detected in serum samples of treated guinea pigs by nitroreductase-proficient strains TA98 and TA98dnp6, but not by... Tipo: Info:eu-repo/semantics/article Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000400026 Identification in silico of putative damage responsive elements (DRE) in promoter regions of the yeast genome Genet. Mol. Biol. Silva,Walkiria Luckwu de Santana; Cavalcanti,Andre Ricardo de Oliveira; Guimarães,Katia Silva; Morais Jr.,Marcos Antonio de. We report an in silico analysis to identify nucleotide sequence motifs in DNA repair genes that may define a binding site for regulatory proteins during the induction of those genes by mutagens. The damage responsive elements (DRE) weight matrix generated in this analysis was used to search for homologous sequences in the promoter region of all genes, including putative gene and hypothetical open reading frames (ORFs), in the Saccharomyces Genome Data Base (SGD). The results demonstrated that over one third of the yeast genes in the database presented at least one 15-bp sequence in their promoter region with 85% or more of similarity to the DRE consensus sequence. The presence of the DRE sequence in the promoter region of regulatory genes and its high... Tipo: Info:eu-repo/semantics/article Palavras-chave: DNA binding site; DNA repair; Gene promoter; Gene regulation; Transfac; Weight matrix. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500025 Production of extracellular lipase by the phytopathogenic fungus Fusarium solani FS1 Rev. Microbiol. Maia,Maria de Mascena Diniz; Morais,Marcia Maria Camargo de; Morais Jr.,Marcos Antonio de; Melo,Eduardo Henrique Magalhães; Lima Filho,José Luiz de. A Brazilian strain of Fusarium solani was tested for extracellular lipase production in peptone-olive oil medium. The fungus produced 10,500 U.l-1 of lipase after 72 hours of cultivation at 25oC in shake-flask at 120 rpm in a medium containing 3% (w/v) peptone plus 0.5% (v/v) olive oil. Glucose (1% w/v) was found to inhibit the inductive effect of olive oil. Peptone concentrations below 3% (w/v) resulted in a reduced lipase production while increased olive oil concentration (above 0.5%) did not further stimulate lipase production. The optimum lipase activity was achieved at pH 8.6 and 30oC and a good enzyme stability (80% activity retention) was observed at pH ranging from 7.6 to 8.6, and the activity rapidly dropped at temperatures above 50oC. Lipase... Tipo: Info:eu-repo/semantics/article Palavras-chave: Enzyme kinetics; Ester hydrolysis; Extracellular lipase; Fusarium solani. Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000400003 Registros recuperados: 3 Primeira ... 1 ... Última

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