|
|
|
|
|
| Huang, Bing; Marchand, Justine; Thiriet-rupert, Stanislas; Carrier, Gregory; Saint-jean, Bruno; Lukomska, Ewa; Moreau, Brigitte; Morant-manceau, Annick; Bougaran, Gael; Mimouni, Virginie. |
| Nitrogen (N) and phosphorus (P) limitations induce neutral lipid accumulation and membrane lipid remodeling in the domesticated oleaginous microalga Tisochrysis lutea. This study compared growth, photosynthetic activity, biochemical and transcriptional responses of T. lutea throughout batch cultures under N or P limitation compared with non-limiting nutrient condition (NP). The results show that, under N limitation, the breakdown and re-use of cellular N-containing compounds contributed to carbohydrates and further triacylglycerol (TAG) accumulation, where the accumulation of docosahexaenoic acid (DHA) in neutral lipids might mainly be derived from the recycling of membrane polar lipids. Conversely, P limitation did not hinder the photosynthetic capacity;... |
| Tipo: Text |
Palavras-chave: Tisochrysis lutea; Phosphorus; Nitrogen; Lipid remodeling; Betaine lipid; Polyunsaturated fatty acid. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00492/60409/64776.pdf |
| |
|
|
| Heydarizadeh, Parisa; Boureba, Wafaa; Zahedi, Morteza; Huang, Bing; Moreau, Brigitte; Lukomska, Ewa; Couzinet-mossion, Aurelie; Wielgosz-collin, Gaetane; Martin-jezequel, Veronique; Bougaran, Gael; Marchand, Justine; Schoefs, Benoit. |
| In this study, we investigated the responses of Phaeodactylum tricornutum cells acclimated to 300 mu mol m(-2) s(-1) photon flux density to an increase (1000 mmol m(-2) s(-1)) or decrease (30 mmol m(-2)s(-1)) in photon flux densities. The light shift occurred abruptly after 5 days of growth and the acclimation to new conditions was followed during the next 6 days at the physiological and molecular levels. The molecular data reflect a rearrangement of carbon metabolism towards the production of phosphoenolpyruvic acid (PEP) and/or pyruvate. These intermediates were used differently by the cell as a function of the photon flux density: under low light, photosynthesis was depressed while respiration was increased. Under high light, lipids and proteins... |
| Tipo: Text |
Palavras-chave: Phaeodactylum tricornutum; Carbon metabolism; Light regulation; Photosynthesis; Chlorophyll fluorescence; Pyruvate hub. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00395/50620/52341.pdf |
| |
|
|
| Huang, Bing; Marchand, Justine; Blanckaert, Vincent; Lukomska, Ewa; Ulmann, Lionel; Wielgosz-collin, Gaëtane; Rabesaotra, Vony; Moreau, Brigitte; Bougaran, Gael; Mimouni, Virginie; Morant-manceau, Annick. |
| Nitrogen (N) and phosphorus (P) limitations induce triacylglycerol (TAG) accumulation and membrane lipid remodelling in the marine diatom Phaeodactylum tricornutum. However, a clear understanding of the metabolic reorientation is still lacking. Carbon partitioning is of great interest because this microalga produces various highly valuable molecules such as lipids and polyunsaturated fatty acids. This study compared growth, photosynthetic activity, biochemical and transcriptional responses of P. tricornutum throughout batch culture under N or P limitation. The integrated results show that the photosynthetic intensity was greatly reduced under N or P limitation. Under N limitation, the degradation and re-use of cellular N-containing compounds contributed to... |
| Tipo: Text |
Palavras-chave: Betaine lipid; Lipid remodelling; Microalga; Nitrogen; Phosphorus; Polyunsaturated fatty acid. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00491/60249/63861.pdf |
| |
|
|
|
