|
|
|
|
|
Vaulot, Daniel; Lepere, Cecile; Toulza, Eve; De La Iglesia, Rodrigo; Poulain, Julie; Gaboyer, Frederic; Moreau, Herve; Vandepoele, Klaas; Ulloa, Osvaldo; Gavory, Frederick; Piganeau, Gwenael. |
Among small photosynthetic eukaryotes that play a key role in oceanic food webs, picoplanktonic Mamiellophyceae such as Bathycoccus, Micromonas, and Ostreococcus are particularly important in coastal regions. By using a combination of cell sorting by flow cytometry, whole genome amplification (WGA), and 454 pyrosequencing, we obtained metagenomic data for two natural picophytoplankton populations from the coastal upwelling waters off central Chile. About 60% of the reads of each sample could be mapped to the genome of Bathycoccus strain from the Mediterranean Sea (RCC1105), representing a total of 9 Mbp (sample T142) and 13 Mbp (sample T149) of non-redundant Bathycoccus genome sequences. WGA did not amplify all regions uniformly, resulting in unequal... |
Tipo: Text |
|
Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00129/23987/21945.pdf |
| |
|
|
Groisillier, Agnes; Herve, Christiane; Jeudy, Alexandra; Rebuffet, Etienne; Pluchon, Pierre Francois; Chevolot, Yann; Flament, Didier; Geslin, Claire; Morgado, Isabel M.; Power, Deborah; Branno, Margherita; Moreau, Herve; Michel, Gurvan; Boyen, Catherine; Czjzek, Mirjam. |
Background: The production of stable and soluble proteins is one of the most important steps prior to structural and functional studies of biological importance. We investigated the parallel production in a medium throughput strategy of genes coding for proteins from various marine organisms, using protocols that involved recombinatorial cloning, protein expression screening and batch purification. This strategy was applied in order to respond to the need for post-genomic validation of the recent success of a large number of marine genomic projects. Indeed, the upcoming challenge is to go beyond the bioinformatic data, since the bias introduced through the genomes of the so called model organisms leads to numerous proteins of unknown function in the still... |
Tipo: Text |
|
Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00011/12219/9003.pdf |
| |
|
|
|