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| Cavada,Benildo Sousa; Vieira,Clébia Crisóstomo; Silva,Lia Magalhães de Almeida; Oliveira,José Tadeu Abreu de; Moreira,Renato de Azevedo. |
| A comparação da metabolização da lectina de Canavalia brasiliensis com as demais proteínas de reservas durante a germinação das sementes na presença de luz foi acompanhada através da combinação de dosagem de proteínas solúveis, determinação da atividade hemaglutinante, cromatografia de afinidade em coluna de Sephadex G-50 e eletroforese em gel de poliacrilamida em presença de dodecilsulfato de sódio (SDS) e 2-mercaptoetanol. Os resultados foram comparados com resultados anteriormente obtidos com sementes germinadas na ausência da luz e ficou evidenciado que, mesmo com as plântuias fotossintetizando, a lectina de Canavalia brasiliensis é preservada, sendo metabolizada após as>demais proteínas de reserva. Estes dados sugerem também que estas proteínas... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lectinas; Canavalia brasiliensis; Germinação. |
| Ano: 1990 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33061990000300003 |
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| Silva,André Luis Coelho da; Caruso,Cecília Sulzbacher; Moreira,Renato de Azevedo; Horta,Ana Cecília Góes. |
| With the objective to promote in vitro callus induction, cotyledon and hypocotyl segments of "perennial soybean" (Glycine wightii (Wight & Arn.) Verdc.) were inoculated in basal medium MS supplemented with sucrose (1.5 e 3%) and 0.8% agar and different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-furfurylaminopurine (kinetin). The explants were maintained in a dark growth room at 28ºC. The best callus induction was observed in explants (cotyledon and hypocotyl) maintained in medium containing the combination of 2,4-D (1 mg.L-1), kinetin (0.1 mg.L-1) and 3% sucrose. To promote callus subculture, the MS medium was supplemented with different combinations of 2,4-D (0.5 to 4.0 mg.L-1), with or without kinetin (0.1 mg.L-1) and sucrose... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glycine wightii; Leguminosae; Callogenesis; Tissue culture. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542003000600011 |
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| Silva,André Luis Coelho da; Caruso,Cecília Sulzbacher; Moreira,Renato de Azevedo; Horta,Ana Cecília Góes. |
| Cotyledon explants were first cultured on MS medium supplemented with 4.52 M 2,4-D and 0.46 mM kinetin. The development of the calli was followed (0, 4, 8, 12, 16, 20, 24, 28 and 32 days after) and the growth curve was determined, based in fresh and dry weight. The growth curve presented sigmoidal form with four distinct phases. The highest growth percentage was observed at the exponential phase and the lowest at the stationary phase. These results indicated that cotyledon callus subculture should be performed 20 days after inoculation. The calli obtained after a period of 28 days were freeze dried, macerated and submitted to extraction with buffers of different pH values (2.6; 4.0; 6.0; 8.0 and 10.0) and the proteins in the extracts were determined by... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glycine; Leguminosae; Callogenesis; SDS-PAGE. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542005000600009 |
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