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	Provedor de dados 74 (2) Autor Morielle-Versute,Eliana (2) Faria,Karina de Cassia (1) Scatena,Mário Pinzan (1) Palavra-chave Chiroptera (2) AluI (1) Archival specimens (1) DAPI (1) DNA extraction (1) FISH (1) Formalin-fixed (1) Telomeric sites (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Info:eu-repo/semantics/other (1) Ano 2008 (1) 2002 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Suitability of DNA extracted from archival specimens of fruit-eating bats of the genus Artibeus (Chiroptera, Phyllostomidae) for polymerase chain reaction and sequencing analysis 74 Scatena,Mário Pinzan; Morielle-Versute,Eliana. To establish a technique which minimized the effects of fixation on the extraction of DNA from formalin-fixed tissues preserved in scientific collections we extracted DNA samples from fixed tissues using different methods and evaluated the effect of the different procedures on PCR and sequencing analysis. We investigated muscle and liver tissues from museum specimens of five species of fruit-eating (frugivorous) bats of the Neotropical genus Artibeus (Chiroptera, Phyllostomidae): A. fimbriatus, A. lituratus, A. jamaicensis, A. obscurus, and A. planirostris. The results indicated that treatment of tissues in buffered solutions at neutral pH and about 37 °C for at least four days improves the quality and quantity of extracted DNA and the quality of the... Tipo: Info:eu-repo/semantics/other Palavras-chave: Archival specimens; Chiroptera; DNA extraction; Formalin-fixed. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100027 In situ hybridization of bat chromosomes with human (TTAGGG)n probe, after previous digestion with Alu I 74 Faria,Karina de Cassia; Morielle-Versute,Eliana. The purpose of this work was to verify the ability of the enzyme Alu I to cleave and/or remove satellite DNA sequences from heterochromatic regions in chromosomes of bats, by identifying the occurrence of modifications in the pattern of fluorescence in situ hybridization with telomeric DNA. The localization and fluorescence intensity of the telomeric DNA sites of the Alu-digested and undigested chromosomes of species Eumops glaucinus, Carollia perspicillata, and Platyrrhinus lineatus were analyzed. Telomeric sequences were detected at the termini of chromosomes of all three species, although, in C. perspicillata, the signals were very faint or absent in most chromosomes. This finding was interpreted as being due to a reduced number of copies of the... Tipo: Info:eu-repo/semantics/article Palavras-chave: AluI; FISH; Chiroptera; Telomeric sites; DAPI. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400003 Registros recuperados: 2 Primeira ... 1 ... Última

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