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Recent developments in nucleic acid based techniques for use in rumen manipulation R. Bras. Zootec.
McSweeney,Christopher; Kang,Seungha; Gagen,Emma; Davis,Carl; Morrison,Mark; Denman,Stuart.
Nucleic acid-based techniques which can be used to characterise complex microbial communities without incubation are now being employed regularly in ruminant nutrition studies. Conventional culture-based methods for enumerating rumen microorganisms (bacteria, archaea, protozoa, and fungi) have been superseded and are now used mainly to obtain pure isolates of novel organisms and reference strains that are required for the development and validation of the nucleic acid approaches. These reference strains are also essential for physiological studies of the lifestyle of the organisms as well as sources of genomic DNA and RNA that can be analysed for functional gene activity. The foundation of the molecular ecology techniques is 16S/18S rDNA sequence analysis...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ecology; Functional analysis; Microbial; Molecular; Ribosomal; Rumen.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982009001300034
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