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Evaluation of glycerin as preserving agent of chicken serum for plate agglutination test Rev. Bras. Ciênc. Avic.
Freitas,ES de; Soldá,A; Calegari,DL; Munaretto,FC; Dietrich,GF; Back,A.
Serum is widely used for the purpose of monitoring and diagnosis support for most of poultry diseases. In the case of the serum plate agglutination test (SPA), commonly used to detect antibodies for Salmonella Pullorum (SP), Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS), serum cannot be frozen because it may result in false positive. Without freezing, serum can last only for a few days. In this experiment, glycerin was evaluated as a serum preservering agent. About 50 samples for each disease and analyzed by SPA test previously were separated. Glycerin was added to serum from commercial chickens, with and without antibodies for SP, MG and MS, in the proportion of 1:1 (serum:glycerin) and kept at refrigerated conditions (2 to 8 ºC). For four...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Seroagglutination; Glycerol; Preservation; Refrigeration; Shelf life.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2014000300015
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