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| Noronha,Marissônia de Araujo; Muniz,Maria de Fátima Silva; Cruz,Marcelo de Menezes; Assunção,Mayara Castro; Castro,José Mauro da Cunha e; Oliveira,Ellen Rebecca Lopes de; Miranda,Camila Gonçalves dos Santos; Machado,Andressa Cristina Zamboni. |
| ABSTRACT: The objective of this study was to accomplish a survey on populations of Meloidogyne and Pratylenchus species in sugarcane farming areas in the state of Alagoas, Brazil. Twenty samples of soil and roots were processed to extract and quantify nematodes; however, the identification of Meloidogyne species was performed using only 12 samples. Pratylenchus spp. were reported at moderate population levels of 68-1556 specimens 50g-1 of roots and 2-298 specimens 100cm-3 of soil in twenty analyzed samples. For Meloidogyne spp., these values were of 12-487 specimens 50g-1 of roots and 0-140 specimens 100cm-3 of soil. Based on electrophoresis of esterase isozymes, M. incognita was reported to be the most frequent species, followed by M. javanica and M.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Field survey; Plant-parasitic nematodes; Saccharum spp. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000200151 |
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| Santana,Jeniffer Keterly Gonçalves; Seixas,André Leal; Ribeiro,Lucas Henrique Gonçalves; Cardoso,Ana Clara Santos; Rocha,Fernando da Silva; Fernandes,Maria de Fátima Gonçalves; Muniz,Maria de Fátima Silva. |
| ABSTRACT: The objective of this research was to evaluate the efficiency of artificial dyes, sunset yellow and red bordeaux S, and the use of glycerol in different concentrations to consistently stain fungal structures in slides containing spores of Oidium sp., Albugo ipomoeae-panduratae, Pochonia chlamydosporia and hyphae of Phytopythium helicoides. Commercial product mixtures of the artificial dyes at 0.5, 1.0, 1.5, 2.0, 3.0 and 5.0% (w/v) added with glycerol at 0.25, 0.5 and 1.0% were evaluated. To stain chlamydospores, the suspension was placed in the staining solution or heated at 80ºC for 5 minutes. The slides were prepared by the wet mount slide method. Fungal spores were consistently stained starting at a concentration of 2% of the staining... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sunset yellow; Red bordeaux; Azo dyes; Fungal staining. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000900750 |
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