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	Provedor de dados BJM (1) Electron. J. Biotechnol. (1) Autor Mustafa,Shuhaimi (2) B. Ariff,Arbakariya (1) Bahreini,Rasoul (1) Dinarvand,Mojdeh (1) Manap,Mohd Yazid Abdul (1) Mardan,Makhdzir (1) Masomian,Malihe (1) Moeini,Hassan (1) Mousavi,Seyed Sadegh (1) Nahavandi,Reza (1) Saari,Nazamid (1) Tajabadi,Naser (1) Mais... Palavra-chave 16S rDNA gene sequences (1) Aspergillus niger ATCC 20611 (1) Culture optimization (1) Honey bee (1) Honey stomach (1) Inulinase (1) Lactobacillus (1) Probiotic (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Journal article (1) Ano 2013 (1) 2012 (1) País Brazil (1) Chile (1) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Effect of extrinsic and intrinsic parameters on inulinase production by Aspergillus niger ATCC 20611 Electron. J. Biotechnol. Dinarvand,Mojdeh; B. Ariff,Arbakariya; Moeini,Hassan; Masomian,Malihe; Mousavi,Seyed Sadegh; Nahavandi,Reza; Mustafa,Shuhaimi. Background: Inulinase is a versatile enzyme from glycoside hydrolase family which targets the β-2, 1 linkage of fructopolymers. In the present study, the effect of medium composition and culture conditions on inulinase production by Aspergillus niger ATCC 20611 was investigated in shake-flasks. Results: The highest extracellular inulinase (3199 U/ ml) was obtained in the presence of 25% (w/v) sucrose, 0.5% (w/v) meat extract, 1.5% (w/v) NaNO3 and 2.5 mM (v/v) Zn2+, at initial pH of 6.5, temperature 35ºC and 6% (v/v) of spores suspension in the agitation speed of 100 rpm. Surfactants showed an inhibitory effect on enzyme production. The optimum temperature for inulinase activity was found to be 50ºC. TLC analysis showed the presence of... Tipo: Journal article Palavras-chave: Aspergillus niger ATCC 20611; Culture optimization; Inulinase. Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000400005 Identification of Lactobacillus plantarum, Lactobacillus pentosus and Lactobacillus fermentum from honey stomach of honeybee BJM Tajabadi,Naser; Mardan,Makhdzir; Saari,Nazamid; Mustafa,Shuhaimi; Bahreini,Rasoul; Manap,Mohd Yazid Abdul. This study aimed to isolate and identify Lactobacillus in the honey stomach of honeybee Apis dorsata. Samples of honeybee were collected from A. dorsata colonies in different bee trees and Lactobacillus bacteria isolated from honey stomachs. Ninety two isolates were Gram-stained and tested for catalase reaction. By using bacterial universal primers, the 16S rDNA gene from DNA of bacterial colonies amplified with polymerase chain reaction (PCR). Forty-nine bacterial 16S rDNA gene were sequenced and entrusted in GenBank. Phylogenetic analysis showed they were different phylotypes of Lactobacillus. Two of them were most closely relevant to the previously described species Lactobacillus plantarum. Other two phylotypes were identified to be closely related to... Tipo: Info:eu-repo/semantics/article Palavras-chave: Honey bee; Honey stomach; Lactobacillus; Probiotic; 16S rDNA gene sequences. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300008 Registros recuperados: 2 Primeira ... 1 ... Última

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