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Virulence profiles of Shiga toxin-producing Escherichia coli and other potentially diarrheagenic E.coli of bovine origin, in Mendoza, Argentina BJM
Pizarro,M.A.; Orozco,J.H.; Degarbo,S.M.; Calderón,A.E.; Nardello,A.L.; Laciar,A.; Rüttler,M.E..
This study described a group of strains obtained from a slaughter house in Mendoza, in terms of their pathogenic factors, serotype, antibiotype and molecular profile. Ninety one rectal swabs and one hundred eight plating samples taken from carcasses of healthy cattle intended for meat consumption were analyzed. Both the swab and the plate samples were processed to analyze the samples for the presence of virulence genes by PCR: stx1, stx2, eae and astA. The Stx positive strains were confirmed by citotoxicity assay in Vero cells. The isolates were subsequently investigated for their O:H serotype, antimicrobial susceptibility and molecular profile by Random Amplification of Polymorphic DNA (RAPD). Twelve E.coli strains were identified by their pathogenicity....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hemolitic Uremic Syndrome (HUS); Shiga Toxin Escherichia coli (STEC); Enteroaggregative Stable toxin (EAST1); Enteropathogenic Escherichia coli (EPEC); Foodborne diseases; Cattle.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400021
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