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| Henneberg,Railson; Nascimento,Aguinaldo José do; Cicchetti,Domenic V; Ferreira,Aline Emmer; Pedrassani,Camila Andrade; Monteiro,Mariana Franco. |
| Los analizadores de hematología ofrecen varios parámetros que se pueden obtener de maneras distintas, en conformidad con el modelo o la marca de los mismos. En el presente trabajo se estudian dos equipos automatizados, analizadores Coulter STKS y Sysmex XE-2100D, y se evalúan de manera comparativa los parámetros del eritrograma en 383 personas elegidas al azar, en el Hospital de Clínicas, de la Universidade Federal do Paraná, Curitiba, Brasil. Todos los parámetros estudiados, excepto la CHCM, presentaron un buen coeficiente de correlación entre los dos equipos. Los parámetros hematocrito (HCT), volumen corpuscular medio (VCM), hemoglobina corpuscular media (HCM) y concentración de hemoglobina corpuscular media (CHCM) presentaron menos de 4% de error entre... |
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Palavras-chave: Hematología; Recuento de células sanguíneas; Automatización; Concentración de hemoglobina corpuscular media; Índices hematimétricos. |
| Ano: 2011 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-29572011000400007 |
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| Higuti,lma Hiroko; Grande,Simone Wichert; Sacco,Roberta; Nascimento,Aguinaldo José do. |
| One hundred and twenty five soil samples were collected from the regions of roots of corn, cassava, potato, bean, sugar cane, soya, and pumpkin. From these, 75 strains were isolated that produced a yellowish halo surrounding the colonies, due to a phenolphtalein-cyclodextrin (CD) complex, and these were selected as alkalophilic CGTase-producing bacteria. All the 75 strains were identified as Bacillus firmus by microscopy and biochemical tests. The activity of the CGTase's varied from 2² to 2(10) dilutions,when assayed by CD-trichloroethylene (TCE)-complex precipitation. Strain 31 that produced the enzyme at the higher level was selected, and its enzyme was partially purified by starch adsorption (x 17) in a yield of 51%. Maximum enzyme activity occurred at... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cyclodextrin glycosyltransferase; Bacillus firmus; Characterization. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000200007 |
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| Higuti,Ilma Hiroko; Silva,Priscila Anunciação da; Papp,Juliana; Okiyama,Vivian Mayumi de Eiróz; Andrade,Edicléia Alves de; Marcondes,Aluizio de Abreu; Nascimento,Aguinaldo José do. |
| Cyclodextrin glycosyltransferase (EC 2.4.1.19, CGTase) production from B. firmus, isolated from soil of Curitiba, PR, was optimized in shake flask using an experimental design approach. The CGTase was produced when the carbon source was starch and beta-CD, but when simple sugars such as glucose, galactose, lactose, sucrose, and maltose were used, there was no enzyme production. CGTase production was the same with either organic nitrogen or inorganic nitrogen source. CGTase activity decreased 2-fold when incubation temperature was increased from 28 to 37 ° C, and decreased 2.1- fold when the initial pH was lowered from 10.3 to 7.4. The colorimetric determinations of alpha - and beta -CD were analyzed as a non-linear relationship and the equilibrium constant... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cyclodextrin glycosyltransferase; Bacillus firmus; Cyclodextrins. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000600001 |
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| Higuti,Ilma Hiroko; Macena,Ivone Rodrigues; Masunari,Setuko; Branco Filho,Mario de Oliveira; Blaskowiski,Marita Maciel Moreira; Nascimento,Aguinaldo José do. |
| The Perequê and Penedo rivers flow through the plains of Praia de Leste and fall into the sea between the municipalities of Pontal do Sul and Ponta do Poço. Both of these rivers belong to the sub-basin of Paranaguá Bay, and are constituent parts of the hydrographic basin of the Atlantic Ocean (State of Paraná). Four sites along the Perequê River (Stations 1-4) and one along the Penedo River (Station 5) were monitorated for water pollution over a 12-month period. During the period of December 1995 to November 1996, three water samples were collected from each site, placed in sterilized bottles and transported to the laboratory in ice-boxes. They were analyzed for the occurrence of total and fecal coliforms, temperature and pH. Fecal pollution was detected... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Total coliforms; Fecal coliforms; Water microbiology. |
| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000400005 |
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| Cogo,Laura Lúcia; Monteiro,Cristina Leise Bastos; Nogueira,Keite da Silva; Palmeiro,Jussara Kasuko; Ribeiro,Marcelo Lima; Camargo,Eloá Ramalho de; Neves,Daniel Locatelli; Nascimento,Aguinaldo José do; Costa,Libera Maria Dalla. |
| Prevalence of H. pylori infection was determined using cultures of gastric biopsy samples of patients attended at the academic hospital of the Federal University of Paraná, Curitiba, Paraná, Brazil. Molecular methods were used to characterize the cagA and vacA genes from bacterial isolates associated with different diseases presented by patients. Out of a total of 81, forty-two gastric biopsy samples tested were positive for H. pylori, with a prevalence of 51.9%. No significant difference was found with regard to the gender (p=0.793) and age (p=0.183) of the patients. Genotype s1m1 vacA gene was found in 67% of the cases of peptic ulcer investigated (p=1.0), despite the limited number of patients with this disease (n=3). A correlation between the presence... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Helicobacter pylori; Virulence genes; CagA; VacA. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400007 |
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| Furman,Aline Emmer Ferreira; Henneberg,Railson; Hermann,Priscila Bacarin; Leonart,Maria Suely Soares; Nascimento,Aguinaldo José do. |
| Sickle cell disease promotes hemolytic anemia and occlusion of small blood vessels due to the presence of high concentrations of hemoglobin S, resulting in increased production of reactive oxygen species and decreased antioxidant defense capacity. The aim of this study was to evaluate the protective action of a standardized extract of Ginkgo biloba (EGb 761), selected due to its high content of flavonoids and terpenoids, in erythrocytes of patients with sickle cell anemia (HbSS, SS erythrocytes) subjected to oxidative stress using tert-butylhydroperoxide or 2,2-azobis-(amidinepropane)-dihydrochloride, in vitro. Hemolysis indexes, reduced glutathione, methemoglobin concentrations, lipid peroxidation, and intracellular reactive oxygen species were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ginkgo biloba; Oxidative stress; Erythrocytes; Azo compounds; Sickle cell anemia. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000400009 |
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