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Nutraceuticals in reproduction of bulls and stallions R. Bras. Zootec.
Arruda,Rubens Paes de; Silva,Daniela Franco da; Alonso,Maria Augusta; Andrade,André Furugen Cesar de; Nascimento,Juliana; Gallego,Andres Mejia; Martins,Simone Maria Massami Kitamura; Granato,Thaís Marques.
The industry has made available in the market a series of substances (nutraceuticals) which intent would be to optimize the use of nutrients in some metabolic paths, influencing positively reproductive performance in animals. However, the response to the use of nutraceuticals varies according to the animal. As the organism is highly complex and in order to achieve a perfect activity of the hypothalamic-pituitary-gonadal axis, an ideal interaction in molecular basis is needed, where the nutraceuticals can have their direct action. The aim of this study was to review the function and research results using the main nutraceuticals (β carotene, vitamin A, L-carnitine, omegas 3, 6 and 9 and Gamma-oryzanol) on reproductive characteristics of bulls and stallions.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Β-carotene bull gamma-oryzanol L-carnitine nutraceutics omegas 3; 6; 9 stallion.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982010001300043
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Damage assessment of the equine sperm membranes by fluorimetric technique BABT
Celeghini,Eneiva Carla Carvalho; Andrade,André Furugen Cesar de; Raphael,Cláudia Fernandes; Nascimento,Juliana; Ticianelli,Janahi Souza; Arruda,Rubens Paes de.
To validate a practical technique of simultaneous evaluation of the plasma, acrosomal and mitochondrial membranes in equine spermatozoa three fluorescent probes (PI, FITC-PSA and MITO) were associated. Four ejaculates from three stallions (n=12) were diluted in TALP medium and split into 2 aliquots, 1 aliquot was flash frozen in liquid nitrogen to induce damage in cellular membranes. Three treatments were prepared with the following fixed ratios of fresh semen: flash frozen semen: 100:0 (T100), 50:50 (T50), and 0:100 (T0). A 150-µL aliquot of diluted semen of each treatment was added of 2 µL of PI, 2 µL of MITO and 80 µL of FITC-PSA; incubated at 38.5ºC/8 min, and sperm cells were evaluated by epifluorescent microscopy. Based in regression analysis, this...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Stallion; Acrosome; Plasma membrane; Mitochondria; Fluorescent probes.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600004
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