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| Signorini-Souza,Isadora de Lourdes; Palacio-Cortés,Angela Maria; Beltrão-Graboski,Tiago; Richardi,Vinicius Sobrinho; Fávaro,Luis Fernando; Navarro-Silva,Mário Antônio. |
| Abstract Histological effects of polybrominated diphenyl ethers (PBDEs) were observed in Chironomus sancticaroli larvae which underwent acute exposure. 2,2′,4-triBDE (BDE-17), 2,2′,4,4′-tetraBDE (BDE-47) and 2,2′,4,4′,5-pentaBDE (BDE-99) were evaluated at 0.5, 2.0 and 20 μg L-1. Cytoplasm vacuolisation of oenocytes was observed in the larvae exposed to BDE-17 and BDE-47. Cuénot cells were disrupted at the brush border as an effect of the three evaluated congeners highlighting BDE-47 at 2.0 μg L-1; 60% of larvae displayed this disruption. The midgut showed changes in the morphology of apex cells located next to the lumen of region I exposed to BDE-17 and BDE-47, while BDE-99 induced a narrowing of the lumen diameter. Significant cytoplasm vacuolisation of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cuénot cells; Flame retardants; Midge; Oenocytes; Salivary glands. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262020000100204 |
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| Aguirre-Obando,Oscar Alexander; Pietrobon,Anna Julia; Bona,Ana Caroline Dalla; Navarro-Silva,Mário Antônio. |
| ABSTRACT After a dengue outbreak, the knowledge on the extent, distribution and mechanisms of insecticide resistance is essential for successful insecticide-based dengue control interventions. Therefore, we evaluated the potential changes to insecticide resistance in natural Aedes aegypti populations to Organophosphates (OP) and Pyrethroids (PY) after chemical vector control interventions. After a Dengue outbreak in 2010, A. aegypti mosquitoes from the urban area of Jacarezinho (Paraná, Brazil) were collected in 2011 and 2012. Insecticide resistance to OP Temephos was assessed in 2011 and 2012 by dose–response bioassays adopting WHO-based protocols. Additionally, in both sampling, PY resistance was also investigated by the Val1016Ile mutation genotyping.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bioassays; Dengue; Insecticide resistance; Knockdown resistance; Pyrethroids; Temephos. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262016000100094 |
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| Richardi,Vinicius Sobrinho; Vicentini,Maiara; Rebechi,Débora; Fávaro,Luis Fernando; Navarro-Silva,Mário Antônio. |
| ABSTRACTChironomidae immature are used as bioindicators of sediment quality in aquatic ecosystems and ecotoxicological assays. Histological descriptions for this family are outdated and limited and there are no studies with Neotropical species. The aim of this study was to describe the tissue architecture of several organs of the larva of Chironomus sancticaroli. For the description of the histological pattern, the larvae were fixed in Duboscq solution for insects at 56 °C, followed by routine histologic processing, infiltration in paraffin, and the sections were stained with Hematoxylin–Eosin. After examining the slides, the tube digestive, salivary gland, excretory, nervous, endocrine, circulatory, and integumentary systems and fat body were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ecotoxicology; Histological biomarker; Sediment. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262015000300240 |
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| Morais,Gisele dos Santos; Pesenti,Emanuele Cristina; Cestari,Marta Margarete; Navarro-Silva,Mário Antônio. |
| Phenanthrene, a Polycyclic Aromatic Hydrocarbon, remains adsorbed to sedimentary particles in aquatic environments. It affects mainly benthic organisms, and is considered potentially genotoxic. In ecotoxicology, species of Chironomus Meigen, 1803 are widely known as bioindicators of the effects of chemicals on aquatic organisms. This study investigates the effects of phenanthrene on the size of the head capsule of Chironomus sancticaroli Strixino & Strixino, 1981 larvae after chronic (eight days) exposure, and DNA damage after acute (96 hours) and chronic exposure (eight days), under laboratory conditions. DNA damage, evaluated using the alkaline comet assay, detected effects for both exposure periods, indicating that phenanthrene is toxic for C.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bioassay; Bioindicador; Development; DNA damage; PHAs. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702014000400003 |
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| Rebechi,Débora; Navarro-Silva,Mário Antônio. |
| Species of Chironomidae are widely used as bioindicators of water quality, since their larvae undergo morphological deformities when in contact with sediment contaminated with chemicals. In this work we endeavored to study the morphology of head structures (antennae, mandible, mentum, pecten epipharyngis, ventromental plate and premandible) throughout the development of the four larval instars of Chironomus sancticaroli Strixino & Strixino, 1981, which can be used in environmental impact analyses. Our results show that it is possible to differentiate among larval instars by doing a quantitative analysis on the number of striae on the ventromental plates. The six structures analyzed changed during larval ontogeny. These changes are part of the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bioindicator; Larva; Mosquito; Morphology. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702012000200009 |
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