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| Nguyen-kim, Hanh; Bouvier, Thierry; Bouvier, Corinne; Ngoc Bui, Van; Le-lan, Huong; Bettarel, Yvan. |
| The periodic rise in seawater temperature is one of the main environmental determinants of coral bleaching. However, the direct incidence of these episodic thermal anomalies on coral-associated microbiota and their subsequent effects on coral health are still not completely understood. In this study, we investigated the dynamics of three main microbial communities of the coral holobiont (e.g., Symbiodinium, bacteria and viruses), during an experimental thermal stress (+4 degrees C) conducted on the scleractinian Fungia repanda. The heat-treatment induced coral bleaching after 11 days and resulted in a final elevation of ca. 9, 130 and 250-fold in the abundance of mucosal viruses, bacteria, and Symbiodinium, respectively. On the contrary, the proportion of... |
| Tipo: Text |
Palavras-chave: Coral bleaching; Thermal stress; Bacteria; Viruses; Holobiont; Vietnam. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00626/73823/73642.pdf |
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| Ngoc Bui, Van; Thu Huyen Nguyen, Thi; Thanh Mai, Chi; Bettarel, Yvan; Yen Hoang, Thi; Thuy Linh Trinh, Thi; Hai Truong, Nam; Ha Chu, Hoang; Thanh Thanh Nguyen, Vu; Duc Nguyen, Huu; Woelfl, Stefan. |
| In Vietnam, a great number of toxic substances, including carcinogens and procarcinogens, from industrial and agricultural activities, food production, and healthcare services are daily released into the environment. In the present study, we report the development of novel yeast-based biosensor systems to determine both genotoxic carcinogens and procarcinogens by cotransformation with two plasmids. One plasmid is carrying human CPR and CYP (CYP3A4, CYP2B6, or CYP2D6) genes, while the other contains the RAD54-GFP reporter construct. The three resulting coexpression systems bearing both CPR-CYP and RAD54-GFP expression cassettes were designated as CYP3A4/CYP2B6/CYP2D6 + RAD54 systems, respectively and used to detect and evaluate the genotoxic potential of... |
| Tipo: Text |
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| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00625/73740/74114.pdf |
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