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	Provedor de dados Genet. Mol. Biol. (2) Autor Nobrega,Francisco G. (2) Back-Brito,Graziella Nuernberg (1) Fonseca,Ghislaine V. (1) Mota,Adolfo Jose (1) Nobrega,Marina P. (1) Santos,Rafael (1) Tambor,José Humberto M. (1) Mais... Palavra-chave Candida palmioleophila (1) Debaryomyces hansenii (1) Differential identification (1) Pichia guilliermondii (1) Real-time PCR (1) Tipo do documento Info:eu-repo/semantics/article (2) Ano 2012 (1) 2001 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Sugarcane genes related to mitochondrial function Genet. Mol. Biol. Fonseca,Ghislaine V.; Tambor,José Humberto M.; Nobrega,Marina P.; Santos,Rafael; Nobrega,Francisco G.. Mitochondria function as metabolic powerhouses by generating energy through oxidative phosphorylation and have become the focus of renewed interest due to progress in understanding the subtleties of their biogenesis and the discovery of the important roles which these organelles play in senescence, cell death and the assembly of iron-sulfur (Fe/S) centers. Using proteins from the yeast Saccharomyces cerevisiae, Homo sapiens and Arabidopsis thaliana we searched the sugarcane expressed sequence tag (SUCEST) database for the presence of expressed sequence tags (ESTs) with similarity to nuclear genes related to mitochondrial functions. Starting with 869 protein sequences, we searched for sugarcane EST counterparts to these proteins using the basic local... Tipo: Info:eu-repo/semantics/article Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100024 Molecular identification of Pichia guilliermondii, Debaryomyces hansenii and Candida palmioleophila Genet. Mol. Biol. Mota,Adolfo Jose; Back-Brito,Graziella Nuernberg; Nobrega,Francisco G.. Traditional phenotypic methods and commercial kits based on carbohydrate assimilation patterns are unable to consistently distinguish among isolates of Pichia guilliermondii, Debaryomyces hansenii and Candida palmioleophila. As result, these species are often misidentified. In this work, we established a reliable method for the identification/differentiation of these species. Our assay was validated by DNA sequencing of the polymorphic region used in a real-time PCR assay driven by species-specific probes targeted to the fungal ITS 1 region. This assay provides a new tool for pathogen identification and for epidemiological, drug resistance and virulence studies of these organisms. Tipo: Info:eu-repo/semantics/article Palavras-chave: Candida palmioleophila; Debaryomyces hansenii; Differential identification; Pichia guilliermondii; Real-time PCR. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100017 Registros recuperados: 2 Primeira ... 1 ... Última

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