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Performance of rapid tests for carbapenemase detection among Brazilian Enterobacteriaceae isolates BJM
Pancotto,Lisiane Rech; Nodari,Carolina Silva; Rozales,Franciéli Pedrotti; Soldi,Tatiane; Siqueira,Carolina Gomes; Freitas,Ana Lúcia; Barth,Afonso Luís.
ABSTRACT The global emergence of carbapenemases led to the need of developing new methods for their rapid detection. The aim of this study was to evaluate the performance of the rapid tests for carbapenemase-producing and non-producing Enterobacteriaceae. Carbapenem non-susceptible Enterobacteriaceae from a surveillance study submitted to a multiplex real time PCR for carbapenemase detection were included in this study. The isolates were subjected to the rapid phenotypic tests Carba NP, Blue-Carba and Carbapenem Inactivation Method (CIM). A total of 83 carbapenemase-producing (43) and non-producing (40) isolates were included in the study. The sensitivity/specificity were 62.7%/97.5%, 95.3%/100%, and 74.4%/97.5% for Carba NP, Blue-Carba and CIM,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Carbapenemases; Rapid tests; Carba NP; Blue-Carba; CIM.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400914
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Draft genome sequence of a GES-5-producing Serratia marcescens isolated in southern Brazil BJM
Nodari,Carolina Silva; Siebert,Marina; Matte,Ursula da Silveira; Barth,Afonso Luís.
Abstract Serratia marcescens is a Gram-negative rod intrinsically resistant to polymyxins and usually associated with wound, respiratory and urinary tract infections. The whole genome of the first GES-5-producing S. marcescens isolated from a Brazilian patient was sequenced using Ion Torrent PGM System. Besides blaGES-5, we were able to identify genes encoding for other β-lactamases, for aminoglycoside modifying enzymes and for an efflux pump to tetracyclines.
Tipo: Info:eu-repo/semantics/article Palavras-chave: GES-5; Serratia marcescens; Whole-genome sequencing.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000200191
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