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	Provedor de dados BJM (1) Electron. J. Biotechnol. (1) Autor Noghabi,Kambiz Akbari (2) Amani,Jafar (1) Aminzadeh,Saeed (1) Daliri,Morteza (1) Ghazizadeh,Elham (1) Ghoroghi,Ahmad (1) Hadi,Faranak (1) Motallebi,Abbasali (1) Mousavi,Amir (1) Safahieh,Alireza (1) Salmanian,Ali Hatef (1) Zarei,Mandana (1) Zolgharnein,Hossein (1) Mais... Palavra-chave Antifungal (1) Brassica napus L. (1) Characterization (1) Chitinase (1) Competitive quantitative PCR (1) Serratia marcescens B4A (1) Transcript level (1) Transgene copy number (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Journal article (1) Ano 2012 (1) 2011 (1) País Brazil (1) Chile (1) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Development of quantitative competitive PCR for determination of copy number and expression level of the synthetic glyphosate oxidoreductase gene in transgenic canola plants Electron. J. Biotechnol. Hadi,Faranak; Salmanian,Ali Hatef; Ghazizadeh,Elham; Amani,Jafar; Noghabi,Kambiz Akbari; Mousavi,Amir. Background: For successful in vitro plant regeneration, plant cell lines with multiple transgene integration and low transgene expression levels need to be ruled out. Although real-time polymerase chain reaction (RT-PCR) is a rapid way to accomplish this, it is also expensive and typically limits the size of the target sequence. Quantitative competitive PCR (QC-PCR) is proven to be a safe and accurate method for determination of both copy number and quantification of transcript levels of synthetic transgenes in transformed plants. Results: The glyphosate oxidoreductase genewas chemically synthesized and used to transform Brassica napus L. via Agrobactrium-mediated transformation. A construct containing the mutated form of a synthetic glyphosate... Tipo: Journal article Palavras-chave: Brassica napus L.; Competitive quantitative PCR; Transcript level; Transgene copy number. Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000400002 Characterization of a chitinase with antifungal activity from a native Serratia marcescens B4A BJM Zarei,Mandana; Aminzadeh,Saeed; Zolgharnein,Hossein; Safahieh,Alireza; Daliri,Morteza; Noghabi,Kambiz Akbari; Ghoroghi,Ahmad; Motallebi,Abbasali. Chitinases have the ability of chitin digestion that constitutes a main compound of the cell wall in many of the phytopathogens such as fungi. In the following investigation, a novel chitinase with antifungal activity was characterized from a native Serratia marcescens B4A. Partially purified enzyme had an apparent molecular mass of 54 kDa. It indicated an optimum activity in pH 5 at 45ºC. Enzyme was stable in 55ºC for 20 min and at a pH range of 3-9 for 90 min at 25ºC. When the temperature was raised to 60ºC, it might affect the structure of enzymes lead to reduction of chitinase activity. Moreover, the Km and Vmax values for chitin were 8.3 mg/ml and 2.4 mmol/min, respectively. Additionally, the effect of some cations and chemical compounds were found to... Tipo: Info:eu-repo/semantics/article Palavras-chave: Antifungal; Characterization; Chitinase; Serratia marcescens B4A. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300022 Registros recuperados: 2 Primeira ... 1 ... Última

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