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Rotavirus infection in a tertiary hospital: laboratory diagnosis and impact of immunization on pediatric hospitalization 55
Pereira,Luciane Aparecida; Raboni,Sonia Mara; Nogueira,Meri B; Vidal,Luine R; Almeida,Sergio Monteiro de; Debur,Maria C; Cruz,Cristina.
BACKGROUND AND OBJECTIVES: Rotavirus (RV) is the main etiological agent of diarrhea in childhood; its laboratory diagnosis is crucial to guide the clinical management and prevention of its spread. RV immunization was introduced in Brazilian 6-month-old children in 2006. The present study was aimed to evaluate three methodologies used for human RV detection in stool samples obtained from patients hospitalized due to gastroenteritis in a teaching hospital and report the impact of RV immunization in hospitalization by diarrhea. METHODS: 293 stool samples collected in the 2001-2008 period were analyzed by enzyme immunoassay (EIA), latex agglutination (LA) and polyacrylamide gel electrophoresis (PAGE). RESULTS: Rotavirus was detected in 34.8% of samples by LA...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rotavirus; Diarrhea; Immunoenzyme techniques; Latex fixation tests; Electrophoresis; Polyacrylamide gel.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000300006
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