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| Issy,A.C.; Castania,V.; Castania,M.; Salmon,C.E.G.; Nogueira-Barbosa,M.H.; Bel,E. Del; Defino,H.L.A.. |
| Animal models of intervertebral disc degeneration play an important role in clarifying the physiopathological mechanisms and testing novel therapeutic strategies. The objective of the present study is to describe a simple animal model of disc degeneration involving Wistar rats to be used for research studies. Disc degeneration was confirmed and classified by radiography, magnetic resonance and histological evaluation. Adult male Wistar rats were anesthetized and submitted to percutaneous disc puncture with a 20-gauge needle on levels 6-7 and 8-9 of the coccygeal vertebrae. The needle was inserted into the discs guided by fluoroscopy and its tip was positioned crossing the nucleus pulposus up to the contralateral annulus fibrosus, rotated 360° twice, and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Intervertebral disc degeneration; Animal models; Histology; Wistar rats. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000300235 |
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| Taveira,A.T.A.; Pereira,F.A.; Fernandes,M.I.M.; Sawamura,R.; Nogueira-Barbosa,M.H.; Paula,F.J.A.. |
| Bone mass loss is a major complication of chronic cholestatic liver disease (CCD). However, the long-term impact of CCD on bone mass acquisition is unknown. We longitudinally assessed bone mineral density (BMD) and factors involved in bone remodeling in 9 children and adolescents with CCD Child-Pugh A (5 boys/4 girls) and in 13 controls (6 boys/7 girls). The groups were evaluated twice, at baseline (T0) and after 3 years (T1), when osteocalcin, deoxypyridinoline, 25-hydroxyvitamin-D, parathyroid hormone, insulin-like growth factor-I (IGF-I), and BMD (L1-L4, proximal femur and total body) were determined. Serum levels of receptor activator for nuclear factor kB ligand (RANKL) and osteoprotegerin were measured only at T1. Lumbar spine BMD was reanalyzed... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hepatic osteodystrophy; Insulin-like growth factor-I; Osteoporosis; Bone mineral density; Bone remodeling. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001100017 |
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