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Normant, Y.. |
Les algues monocellulaires utilisées comme nourriture par les larves de bivalves et de pénéides doivent répondre à plusieurs critères et principalement : multiplication rapide, tailles assez petites pour être ingérées, cultures ne formant pas d'amas. Ce présent rapport décrit l'unité de production contrôlée d'algues au Centre Océanologique du Pacifique, la méthodologie appliquée pour les différentes algues, les nouveaux milieux mis au point. |
Tipo: Text |
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Ano: 1978 |
URL: http://archimer.ifremer.fr/doc/00428/53988/55145.pdf |
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Flassch, Jean-pierre; Salaun, G.; Normant, Y.. |
The Prasinophyceae Tetraselmis suecica Butcher, was cultivated at a density of 2 to 3 million cells per ml and was concentrated with a decanting centrifugal extractor at 1 m3/hr flow. The samples, obtained in the form of a paste, were diluted to 50 x 10(6) cells per ml and mixed with two cryoprotective agents such as glycerol (10, 20, 30%) and dimethyl sulfoxide (DMSO, 5, 10, 15%), then frozen and stored a t -25 C. After a 1 month period, results by decreasing order of viability areas follows : glycerol, l0%, 20%, DMSO 15%, glycerol 10%, a starting culture of 0.69 x 10(6) cells per ml in 500 ml sea water enriched with Conway stock solution reached 5.49 x 10(6) cells per ml culture a t the end of 4 days. The aim of these experiments was to obtain a good... |
Tipo: Text |
Palavras-chave: Histoire Ifremer. |
Ano: 1975 |
URL: http://archimer.ifremer.fr/doc/1975/publication-5081.pdf |
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Flassch, Jean-pierre; Normant, Y.. |
The start of the mollusc, crustacean and fish artificial production programs required the creation of a room producing 300 litres of algal culture daily over 6 days, but able to reach 400 litres 3 days a week. The cultures are monospecific; 98 % of the produced algae are Prasinophyceae, Tetraselmis suecica Butcher (Parke and Dixon, 1964), a green flagellate alga measuring eight to ten [ ?] at its longest length. |
Tipo: Text |
Palavras-chave: Histoire Ifremer. |
Ano: 1973 |
URL: http://archimer.ifremer.fr/doc/1973/publication-5979.pdf |
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