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The effect of concanavalin A on the replication of rotavirus (SA-11) in cell culture BABT
Hasenack,Beatriz S.; Botelho,Maria Valéria J.; Lauretti,Flávio; Melo,Fernando L. de; Orlandi,Janaina M.; Linhares,Rosa Elisa C.; Ueda,Marli; Nozawa,Carlos M..
Rotavirus (RV) strain SA-11 was studied with respect to its infectivity in MA-104 cell cultures and the effect of concanavalin A (ConA). Receptors for ConA at the surface of MA-104 cell were determined by fluorescence assay and specifically inhibited by D-mannose. The kinetics of virus growth was carried out by plaque assay. Electron microscopy and polyacrylamide gel electrophoresis were used for monitoring the experiments. It was concluded that RV replication was not affected consistently by ConA, however it interfered with the development of cytopathic effect (CPE) without altering virus yields.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rotavirus; Concanavalin A; Cell culture.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000200003
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Strains of Lentinula edodes suppress growth of phytopathogenic fungi and inhibit Alagoas serotype of vesicular stomatitis virus BJM
Sasaki,Selma H.; Linhares,Rosa E.C.; Nozawa,Carlos M.; Montalván,Ricardo; Paccola-Meirelles,Luzia D..
Four Lentinula edodes strains (Le10, 46, K2, Assai) were assessed for their antagonistic effect on four filamentous fungus species of agricultural importance (Helminthosporium euphorbiae, Helminthosporium sp, Fusarium solani and Phomopsis sojae) and on Alagoas serotype of Vesicular Stomatitis Virus (VSA). The L. edodes strains studied had variable effects on the filamentous fungi and on VSA. The K2 and Le10 strains were antagonistic on the fungi assessed and the 46 and K2 strains were efficient on the Vesicular Stomatitis Virus. The results widened the list of beneficial effects of L. edodes on the control and prevention of animal pathogenic virus and filamentous fungi.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phytopathogenic fungi; Shiitake; Inhibition; Vesicular Stomatitis Virus (Alagoas).
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000100012
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