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| Salem,Julia Ignez; Carvalho,Clarice Maia; Ogusku,Mauricio Morishi; Maia,Rosália; Ruffino-Netto,Antonio. |
| We elaborated an alternative culture method, which we denominated PKO (initials in tribute of respect to Petroff, Kudoh and Ogawa), for isolating Mycobacterium tuberculosis from sputum for diagnosis of pulmonary tuberculosis (TB), and to compare its performance with the Swab and Petroff methods. For the technique validation, sputum samples from patients suspected of pulmonary TB cases were examined by acid-fast microscopy (direct and concentrated smear), PKO, Swab and Petroff methods. We found that Petroff and PKO methods have parity in the effectiveness of M. tuberculosis isolation. However, by the PKO method, 65% of isolated strains were detected in a period of <FONT FACE=Symbol>£</FONT>15 days, while by the Petroff method the best detection... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Diagnostic method; Mycobacterium tuberculosis; PKO; Tuberculosis. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672007000300013 |
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| Restrepo,Adriana Vallejo; Salem,Julia Ignez; Ogusku,Mauricio Morishi; Gomes,Lucia de Fátima; Fraiji,Nelson Abrahim. |
| Investigou-se por métodos bacteriológicos (cultivo) e moleculares (PCR - Restriction Enzyme Analysis, PRA), a presença de micobactérias ambientais em águas de torneira, soluções e luvas cirúrgicas, utilizadas nas etapas dos procedimentos cirúrgicos executados no centro cirúrgico do Hospital Universitário Getulio Vargas (HUGV), na cidade de Manaus-Amazonas/Brasil. Foram colhidas e analisadas 105 amostras sendo: 24 de águas (colhidas das 2 torneiras existentes no centro cirúrgico), 8 de solução de Povidine e 7 de solução de Clorhexidina, que servem para a higienização das mãos dos cirurgiões; 39 de luvas cirúrgicas (superfícies internas e externas); e 27 de soluções que foram efetivamente utilizadas durante o ato cirúrgico. Por método bacteriológico... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Micobactérias Ambientais; MNT; Água; Soluções anti-sépticas; Luvas; Procedimentos cirúrgicos. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672009000400017 |
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| Sadahiro,Aya; Moraes,José Roberto Feresin; Moraes,Maria Elisa Hue; Romero,Matilde; Gouvea,Nancy Alves de Lima; Gouvea,Celso José; Ogusku,Mauricio Morishi; Campbell,Iphis; Zaitz,Clarisse. |
| The frequency of HLA (Human Leucocyte Antigens) was analyzed in 25 non-consanguineous Brazilian Ashkenazic Jews, resident in the city of São Paulo, Brazil, suffering from chronic dermatophytosis caused by T. rubrum, and in 25 non-infected individuals belonging to the same ethnic group. Statistically significant values (p<0.05) were observed for HLA-B14 associated with resistance to chronic dermatophytosis and HLA-DQB1*06 (p=0.05) possibly related to susceptibility. These findings suggest that genes on the chromosome 6, in the region of the major histocompatibility complex, may influence the development of chronic dermatophytosis. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chronic dermatophytosis; Trichophyton rubrum; (HLA) human leucocyte antigens. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100011 |
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| Ogusku,Mauricio Morishi; Sadahiro,Aya; Hirata,Mário Hiroyuki; Hirata,Rosário D. C.; Zaitz,Clarisse; Salem,Júlia Ignez. |
| Seeking to improve the laboratory diagnosis of Cutaneous Tuberculosis, a study was carried out on the application of PCR technique in macerated, decontaminated(with 4% H2SO4 for elimination of normal microbiot), neutralized (with 4% NaOH)biopsies tissues samples stored at -20ºC. Of the 37 samples submitted for study, 16.22% were positive by microscopy for acid-fast bacilli (concentrated method) and in 43.24% the Mycobacterium tuberculosis was isolated in Löwenstein-Jensen medium. Using a M. tuberculosis complex specific primer set (gene sequence for 16S rDNA), the mycobacterial DNA was detected in 24.32% of the biopsies. The sensitivity and specificity of PCR were 43.7% and 90.4%, respectively. Due to low sensitivity and discrepant results between... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mycobacterium tuberculosis; Cutaneous tuberculosis; Polymerase Chain Reaction (PCR); Laboratory diagnosis. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000200015 |
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