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| Registros recuperados: 11 | |
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| Gamon, T. H. M.; Batista, H. B. C. R.; Cruz, F. P. N.; Peixoto, Z. M. P.; Carnieli Jr, P.; Oliveira, R. N.; Nasruai, A. C. R.; Castilho, J. G.. |
| A cinomose é uma doença viral altamente contagiosa que afeta o sistema respiratório, o sistema gastrointestinal e o sistema nervoso central (SNC) dos caninos e felinos. Devido à grande diversidade de sinais clínicos observados nos animais afetados, a confirmação laboratorial é fundamental para o diagnóstico definitivo da doença. Este trabalho teve por objetivo comparar as técnicas utilizadas no diagnóstico laboratorial da cinomose canina. Para tanto foram selecionadas 20 amostras de SNC de caninos domésticos, enviados ao Laboratório de Virologia da Seção de Diagnóstico da Raiva do Instituto Pasteur. Destas amostras, 7 foram provenientes de animais sem histórico clinico de doença e 13 foram provenientes de animais com manifestação de sinais clínicos ... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/16287 |
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| Scheffer, K. C.; Fahl, W. O.; Iamamoto, K.; Carnieli Jr., P.; Carrieri, M. L.; Oliveira, R. N.; Ito, F. H.. |
| Molecular techniques have been used increasingly as tools for the diagnosis by detecting the rabies virus genome. This study aimed to detect the presence of rabies virus in the wash of skull and in different organs of the genus Artibeus bats using the hemi-nested RT-PCR (hnRT-PCR) and Real Time RT-PCR molecular techniques. From approximately 4,000 specimens of bats received at the Institute Pasteur for rabies diagnosis, 30 bats of the genus Artibeus were selected, with records of positive results for rabies by the traditional techniques of direct fluorescent antibody test (FAT) and inoculation of murine neuroblastoma cell line (N2A). Salivary glands, urinary bladders, kidneys, lungs, and also the washes of the skullcaps of the specimens were collected. The... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/3105 |
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| Castilho, J. G.; Batista, H. B. C. R.; Rodrigues, A. C.; Carnieli Jr, P.; Oliveira, R. N.; Silva, A. C. R.; Caporale, G. M. M.; Carrieri, M. L.; Kotait, I.; Brandão, P. E.. |
| The aim of this study was to develop a new mechanism for antiviral therapy against rabies based on the introduction by transfection with a cationic reagent (lipofectamine 2000) of antibodies into neuronal cells infected with the rabies virus. N2A cells were infected using 96-well plates and different viral concentrations (0.1, 1.0, 10 and 100 TCID50) of three lineages of rabies virus circulating in Brazil (dog, Desmodus rotundus and Eptesicus furinalis). After incubation for 24 h, the cells were transfected with antirabies- virus polyclonal antibodies and lipofectamine 2000. These cells made up the treatment group (TG). The cells in the negative control group (CG) were treated with only Minimum Essential Medium. After 11 hours, the plates were fixed with... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/3080 |
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| Castilho, J. G.; Rodrigues, A. C.; Carnieli, P. J. R.; Oliveira, R. N.; Silva, A. C. R.; Caporale, G. M. M.; Brandão, P. E.; Batista, H. B. C. R.. |
| This study describes the comparison of in vivo and in vitro transfection of antibodies to use as a new mechanism for antiviral therapy against rabies. The transfection was made in vitro with neuroblastoma murine cell line (Neuro‐2a) and in vivo with 21 years old mice. Both cells and mice were infected with different infectious doses (0.1; 1.0; 10 and 100 ID50) of the rabies virus. After 24 hours the infected cells and mice were transfected with polyclonal antibodies against rabies produced in equines (unpurified hyperimmune serum ‐ total antibody) using a cationic reagent, the lipofectamine 2000 previously diluted 1:20, for the negative transfection control, only minimum essential medium was used. The ability of the antibodies to neutralize the rabies... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/17374 |
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| Carnieli Jr., P.; Batista, H. B.C.R.; Schereffer, K. C.; Fahl, W. O.; Lima, J. Y.O.; Oliveira, R. N.; Castilho, J. G.; Iamamoto, K.; Carrieri, M. L.; Kotait, I.. |
| The identification of animal species that transmit pathogens such as the rabies virus is of the utmost importance for public health and the natural history of infectious and contagious diseases. Diagnostic laboratories very often receive mauled or decomposing animal carcasses, particularly of bats, rendering morphometric identification unviable. The existence of different regional names for the same animal, morphological variability and the lack of staff trained in zoological identification constitute a serious problem for epidemiological surveillance. Molecular techniques are used routinely and effectively in systematics, evolution and ecology to identify species and can even be used to identify hybrids that originated from genetically close animals, in... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/3106 |
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| Carrieri, M. L.; Achkar, S. M.; Castilho, J. G.; Oliveira, R. N.; Carnieli Jr, P.; Macedo, C. I.; Omoto, T. M.; Takaoka, N. Y.; Kotait, I.. |
| The São Paulo State Rabies Control Program, coordinated by the Pasteur Institute of São Paulo, has been involved in endeavors to improve the epidemiological surveillance of rabies in dogs, cats and bats, particularly in urban areas. Since 1998, when the last case of canine rabies due to antigenic variant 2 occurred in the state, the Institute has received 66,352 canine central nervous system samples, 17,796 samples from cats and 38,133 from bat specimens. Using conventional techniques for rabies diagnosis, 11 dogs, 8 cats and 554 bats were found to be positive for this disease. When molecular techniques, such as antigenic typing and genetic sequencing, were used, antigenic variant 3, which is associated with Desmodus rotundus, was detected in 11 dogs and 7... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/2566 |
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| Carnieli Jr, P.; Oliveira, R. N.; Castilho, J. G.; Vieira, L. F. P.. |
| Antigenic variant 2 of the Rabies Virus (RABV), typically found in dogs in Brazil, still circulates in the North and Northeast of Brazil in spite of mass vaccination campaigns against rabies in domestic animals. Previous studies have shown that this variant is made up of two main genetic lineages: one found in dogs (Canis familiaris) and another typically found in wild canids, particularly the crab-eating fox (Cerdocyon thous), which is known in Brazil as cachorro do mato. C. thous is currently the only wild land species in which RABV is continually isolated, with an average of 20 cases/year. Although previous studies and phylogenetic analyses of the N and G genes and intergenic G-L region all confirmed the existence of these two genetic lineages, the way... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/3102 |
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| Scheffer, K. C.; Fahl, W. O.; Lamamoto, K.; Carnieli Junior, P.; Carrieri, M. L.; Mori, E.; Oliveira, R. N.; Ito, F. H.. |
| The aim of this study was to detect the rabies virus (RABV) presence in different organs of frugivorous bats using molecular techniques such as RT‐PCR, hnRT‐PCR, and the Real Time RT‐PCR. Thirty bats of the genus Artibeus were selected and resulted as positive by the DFA test and N2A‐cells inoculation test using brain tissue in both tests. Samples of salivary gland tissue, urinary bladder tissue, kidney tissue, lung tissue, stool, and skull lavage were collected for molecular assays. The organs and the stool were diluted at 1:10 (w/v) and the urinary bladder was diluted at 1:20 (w/v). The RT‐PCR and the hnRT‐PCR were performed using specific nucleoprotein gene‐target primers. The product obtained by reverse transcription technique was submitted to the Real... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/17385 |
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| Martorelli, L. F. A.; Kataoka, A. P. A. G.; Campos, A. C. A.; Sobre, M. M.; Araujo, D. B.; Rosa, A. R.; Trezza Netto, J.; Oliveira, R. N.; Almeida, M. F.; Sacramento, D. V. R.; Durigon, E. L.; Favoretto, S. R.. |
| Rabies was detected in two bats colonies of insectivorous Histiotus velatus that used as shelter ceilings of buildings in the same park in the North region of São Paulo City, Brazil in 2001 and 2009. This park although located in an urban area, has a large area of rainforest. Rabies diagnosis was made by FAT (Fluorescent Antibody Test) and MIT (Mouse Inoculation Test) techniques. The number of bats submitted to rabies diagnosis was forty-three in the first colony and forty in the second. Before it, bats received anesthesic and blood samples were collected by cardiac puncture in sixty animals (thirty-eight of the first colony and twenty-two of the second colony) and the presence of rabies virus neutralizing antibodies was determined by SFIMT (Simplified... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/2550 |
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| Mori, E.; Scheffer, K. C.; Fahl, W. O.; Oliveira, R. N.; Asano, K. M.; Carnieli Jr, P.; Carrieri, M. L.; Kotait, I.. |
| Human rabies is still an important public health problem in some Brazilian regions. Usually, ante-mortem diagnosis of rabies is made by demonstration of virus antigen by direct immunofluorescence in corneal or conjunctival smears and skin biopsies; however, this technique has a low sensitivity. Recently, molecular techniques such as the reverse transcriptase polymerase chain reaction (RT-PCR) and nucleic acid sequence based amplification assay (NASBA) have been developed to improve the sensitivity and specificity of ante as well as post-mortem diagnosis of rabies. Rapid and accurate diagnosis of ante-mortem human rabies is essential for effective medical management and to ensure appropriate post-exposure prophylaxis of potential contacts with the patient.... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/3098 |
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| Batista, H. B. C. R.; Carnieli Júnior, P.; Oliveira, R. N.; Castilho, J. G.. |
| Rabies is a worldwide zoonotic disease caused by rabies virus (RABV). According to the World Health Organization, more than 55,000 people still die of rabies every year. The domestic dogs are the major responsible for transmission of the virus for people.The human rabies transmitted by dog is considered a neglected disease. Rabies can be controlled by correct prophylaxis, but since the first case of the rabies cure, different studies have been made to search potential mechanisms of rabies treatment. This work was carried out in order to examine the toxicity and the efficiency of "in vivo" transfection of antibodies against rabies. In this study mice were infected by intracerebral route with 100, 10, 1 and 0,1 lethal doses (LD50) of RABV isolated from dog.... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/17377 |
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| Registros recuperados: 11 | |
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