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	Provedor de dados Electron. J. Biotechnol. (1) Autor Guimarães,Luis Henrique Souza (1) Jorge,João Atílio (1) Oliveira,Arthur Henrique Cavalcanti de (1) Rustiguel,Cynthia Barbosa (1) Terenzi,Héctor Francisco (1) Palavra-chave ?-D-fructofuranosidase (1) Agroindustrial substrate (1) Aspergillus (1) Solid state fermentation (1) Sucrose (1) Tipo do documento Journal article (1) Ano 2011 (1) País Chile (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Biochemical properties of an extracellular β-D-fructofuranosidase II produced by Aspergillus phoenicis under Solid-Sate Fermentation using soy bran as substrate Electron. J. Biotechnol. Rustiguel,Cynthia Barbosa; Oliveira,Arthur Henrique Cavalcanti de; Terenzi,Héctor Francisco; Jorge,João Atílio; Guimarães,Luis Henrique Souza. The filamentous fungus A. phoenicis produced high levels of ?-D-fructofuranosidase (FFase) when grown for 72 hrs under Solid-State Fermentation (SSF), using soy bran moistened with tap water (1:0.5 w/v) as substrate/carbon source. Two isoforms (I and II) were obtained, and FFase II was purified 18-fold to apparent homogeneity with 14% recovery. The native molecular mass of the glycoprotein (12% of carbohydrate content) was 158.5 kDa with two subunits of 85 kDa estimated by SDS-PAGE. Optima of temperature and pH were 55�C and 4.5. The enzyme was stable for more than 1 hr at 50�C and was also stable in a pH range from 7.0 to 8.0. FFase II retained 80% of activity after storage at 4�C by 200 hrs. Dichroism analysis showed the presence of random... Tipo: Journal article Palavras-chave: Agroindustrial substrate; Aspergillus; ?-D-fructofuranosidase; Solid state fermentation; Sucrose. Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000200002 Registros recuperados: 1 Primeira ... 1 ... Última

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