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| Registros recuperados: 13 | |
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| Smaniotto,Alessandra; Skovronski,Aline; Rigo,Elisandra; Tsai,Siu Mui; Durrer,Ademir; Foltran,Lillian Liva; Di Luccio,Marco; Oliveira,J. Vladimir; Oliveira,Débora de; Treichel,Helen. |
| The lipase produced by a newly isolate Sporidiobolus pararoseus strain has potential catalysis ability for esterification reactions. In order to improve its synthetic activity, this work aimed at optimizing 'synthetic lipase' production by submerged fermentation of a conventional media based on peptone, yeast extract, NaCl and olive oil using experimental design technique. According to the results obtained in the first experimental design (2(4-1)), yeast extract and NaCl concentrations were tested to further optimization by response surface methodology. The maximum 'synthetic lipase' activity obtained was 26.9 U/mL in the optimized media (5.0, 6.8, 7.0 and 1.0% (wt/v) of peptone, yeast extract, NaCl and olive oil, respectively), representing a 6.36-fold... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sporidiobolus pararoseus; Experimental design; 'synthetic lipase'; Submerged fermentation. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400033 |
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| Smaniotto,Alessandra; Skovronski,Aline; Rigo,Elisandra; Tsai,Siu Mui; Durrer,Ademir; Foltran,Lillian Liva; Paroul,Natália; Luccio,Marco Di; Oliveira,J. Vladimir; Oliveira,Débora de; Treichel,Helen. |
| Lipases produced by a newly isolated Sporidiobolus pararoseus strain have potential catalytic ability for esterification reactions. After production, the enzymatic extracts (conventional crude and precipitated, 'CC' and 'CP', and industrial crude and precipitated, 'IC' e 'IP') were partially characterized. The enzymes presented, in general, higher specificity for short chain alcohols and fatty acids. The precipitated extract showed a good thermal stability, higher than that for crude enzymatic extracts. The 'CC' and 'CP' enzymes presented high activities after exposure to pH 6.5 and 40 ºC. On the other hand, the 'IC' and 'IP' extracts kept their activities in a wide range of pH memory but presented preference for higher reaction temperatures. Preliminary... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sporidiobolus pararoseus; 'synthetic lipase'; Submerged fermentation; Enzyme characterization. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100043 |
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| Beltrame,Cezar Augusto; Kubiak,Gabriela Busnello; Lerin,Lindomar Alberto; Rottava,Ieda; Mossi,Altemir José; Oliveira,Débora de; Cansian,Rogério Luis; Treichel,Helen; Toniazzo,Geciane. |
| The efficiency of four Sanitizers - peracetic acid, chlorhexidine, quaternary ammonium, and organic acids - was tested in this work using different bacteria recognized as a problem to meat industry, Salmonella sp., S. aureus, E. coli and L. monocytogenes. The effects of sanitizer concentration (0.2, 0.5, 0.6, 1.0, 1.1 and 1.4%), at different temperatures (10 and 45 °C) and contact time (2, 10, 15, 18 and 25 minutes) were evaluated. Tests in an industrial plant were also carried out considering previously obtained results. In a general way, peracetic acid presented higher efficiencies using low concentration (0.2%) and contact time (2 minutes) at 10 °C. The tests performed in industrial scale showed that peracetic acid presented a good performance in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sanitizers; Bacteria; Food industries. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612012000200003 |
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| Kempka,Aniela Pinto; Krüger,Roberta Letícia; Valduga,Eunice; Di Luccio,Marco; Treichel,Helen; Cansian,Rogério; Oliveira,Débora de. |
| Atualmente, são lançados no mercado diversos novos produtos, sendo que, especificamente na área de laticínios, ênfase tem sido dada ao desenvolvimento de produtos funcionais. Concernente à amplitude deste tema, o presente trabalho possui como objetivo geral o desenvolvimento de uma bebida láctea fermentada com características probióticas. Além disso, propõe-se a utilização de soro de leite e extrato hidrossolúvel de soja como substratos, agregando valor às matérias-primas de baixo custo, com características funcionais apontadas na literatura. Para tal, utilizou-se a técnica do planejamento de experimentos para obter a formulação de bebida láctea, utilizando como substratos leite de vaca, soro de leite e extrato hidrossolúvel de soja. A formulação contendo... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bebida láctea; Formulação; Probiótico; Bifidobacterium. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612008000500027 |
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| Lerin,Lindomar; Toniazzo,Geciane; Oliveira,Débora de; Rottava,Leda; Dariva,Cláudio; Cansian,Rogério Luis; Treichel,Helen; Padilha,Francine; Antunes,Octávio Augusto Ceva. |
| Limonene is a monoterpene obtained in large amounts from essential oils and is used as a raw material for the synthesis of flavors and fine chemicals. Several pathways or routes for the microbial degradation of limonene making use of the cytochrome P450-dependent monooxygenases have been described. In this study, we present a fermentative screening of microorganisms in order to verify their ability to perform the desirable conversion. In parallel, the PCR technique was used to select the microorganisms that contain the limC gene, which is responsible for the conversion of carveol to carvone. The microorganisms selected by PCR were not able to bioconvert limonene. From this result, we can suppose that these strains do not have the gene that codifies the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: CDH (carveol dependent dehydrogenase); Carveol; Carvone; PCR; Fermentative screening. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612010000200017 |
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| Pinheiro,Thaís da Luz Fontoura; Menoncin,Silvana; Domingues,Natália Molossi; Oliveira,Débora de; Treichel,Helen; Di Luccio,Marco; Freire,Denise Maria Guimarães. |
| The growing interest in lipase production is related to the potential biotechnological applications that these enzymes present. Current studies on lipase production by submerged fermentation involve the use of agro-industrial residues aiming at increasing economic attractiveness. Based on these aspects, the objective of this work was to investigate lipase production by Penicillium verrucosum in submerged fermentation using a conventional medium based on peptone, yeast extract, NaCl and olive oil, and an industrial medium based on corn steep liquor, Prodex Lac (yeast hydrolysate), NaCl and olive oil, as well as to characterize the crude enzymatic extracts obtained. Kinetics of lipase production was evaluated and the highest enzymatic activities, of 3.15 and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lipases; Penicillium verrucosum; Submerged fermentation; Partial characterization. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612008000200028 |
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| Menoncin,Silvana; Domingues,Natalia Molossi; Freire,Denise Maria Guimarães; Oliveira,José Vladimir; Di Luccio,Marco; Treichel,Helen; Oliveira,Débora de. |
| O principal interesse em imobilizar uma enzima é obter um biocatalisador com atividade e estabilidade que não sejam afetadas durante o processo, em comparação à sua forma livre. Aliado ao potencial biotecnológico que as lipases apresentam, a aplicação destas em nível industrial requer a investigação de técnicas viáveis para reutilização e aumento da estabilidade, conferindo relevância aos processos de imobilização. Neste trabalho investigou-se a imobilização da lipase produzida por fermentação em estado sólido utilizando Penicillium verrucosum em dois suportes hidrofóbicos; Accurel EP 1000 e Carvão Ativo. Para a imobilização das lipases foi adicionado 1 g de suporte a 50 mL de uma solução enzimática, estes permaneceram em contato por 2 horas em banho de... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lipases; Penicillium verrucosum; Imobilização. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612009000200033 |
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| Hildebrand,Cristiane; Dalla Rosa,Clarissa; Freire,Denise Maria Guimarães; Destain,Jaqueline; Dariva,Cláudio; Oliveira,Débora de; Oliveira,José Vladimir. |
| The objective of this work is to investigate the production of fatty acid ethyl esters from soybean oil in compressed propane using a non-commercial lipase from Yarrowia lipolytica and two commercial ones as catalysts, Amano PS and Amano AY30. The experiments were performed in the temperature range of 35-65 °C. at 50 bar, enzyme concentration of 5 wt%, oil to ethanol molar ratio of 1:6 and 1:9, and solvent to substrates mass ratio of 2:1 and 4:1. The results indicated that low reaction conversions were generally obtained with the use of commercial and non-commercial lipases in pressurized propane medium. On the other hand, the aspects of low solvent to substrates mass ratio and mild temperature and pressure operating conditions used to produce ethyl esters... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biodiesel; Fatty acid ethyl esters; Lipase; Propane; Soybean oil. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612009000300023 |
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| Cansian,Rogério Luis; Mossi,Altemir José; Oliveira,Débora de; Toniazzo,Geciane; Treichel,Helen; Paroul,Natalia; Astolfi,Viviane; Serafini,Luciana Atti. |
| Este trabalho teve como objetivo determinar a atividade antimicrobiana e antioxidante do óleo essencial de Ho-Sho. O principal componente do óleo essencial obtido a partir de folhas da planta submetidas ao processo de hidrodestilação foi o linalol (80 a 95% m/m). O óleo essencial mostrou atividade antimicrobiana para todos os microrganismos testados, com exceção de Pseudomonas aeruginosa. A maior atividade antimicrobiana do óleo essencial sobre as bactérias testadas foi observada sobre Xanthomonas campestris (33,0 mm) e a menor sobre Yersinia enterocolitica (10,5 mm). Para a concentração inibitória mínima (CIM), observou-se que todos os microrganismos apresentaram-se susceptíveis ao óleo essencial de Ho-Sho. A variação das CIM para as bactérias... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Atividade antimicrobiana; Atividade antioxidante; Cinnamomum camphora. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612010000200014 |
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| Esmelindro,Maria Carolina; Toniazzo,Geciane; Waczuk,Adroaldo; Dariva,Cláudio; Oliveira,Débora de. |
| A erva-mate é uma matéria-prima de grande importância para a região Sul do Brasil, sendo que a produção anual é de aproximadamente 650.000 toneladas de folhas. Atualmente, problemas com o excesso de oferta têm incentivado pesquisadores e empresários a buscar alternativas para a utilização da erva-mate como matéria-prima para o desenvolvimento de novos produtos bem como promover melhorias no processamento industrial visando a obtenção de características organolépticas desejáveis. Neste sentido, o presente trabalho teve por objetivo realizar a caracterização físico-química da erva-mate em função das etapas do processamento industrial (sapeco, secagem e tempo de cancheamento) e verificar como estas etapas influem nos teores de cinzas, fibras, gorduras,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Erva-mate; Processamento industrial; Caracterização físico-química. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612002000200016 |
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| Dalla Rosa,Clarissa; Oliveira,Débora de; Oliveira,José Vladimir. |
| This research note addresses the role of organic solvent amount in the production of fatty acid ethyl esters from soybean oil. N-hexane was chosen as solvent and two commercial immobilized lipases as catalysts, Novozym 435 and Lipozyme IM. The reactions were conducted in 6 hours, varying the solvent to oil ratio from zero to 50 (v/wt) and adopting adopting for Novozym 435: 65 ºC, enzyme concentration (E, wt%) = 5, oil to ethanol molar ratio (R) = 1:10, water addition (H, wt%) = 0, and for Lipozyme IM: 35 ºC, E = 5 wt%, R = 1:3, H = 10 wt%. For Lipozyme IM, an increase in solvent amount is shown to lead to an enhancement of reaction conversion, while a negligible effect was found for Novozym 435. When using 30 mL of solvent the reaction conversions were 88%... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biodiesel; Lipases; Organic solvent; Soybean oil. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612010000100011 |
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| Silva,Adriane da; Zitkoski,João; Mazutti,Marcio Antônio; Mossi,Altemir; Oliveira,José Vladimir; Oliveira,Débora de; Cichoski,Alexandre José; Treichel,Helen. |
| This work reports the use of experimental design for the assessment of the effects of process parameters on the production of fish nuggets in an industrial scale environment. The effect of independent factors on the physicochemical and microbiological parameters was investigated through a full 24 experimental design. The studied factors included the temperature of fish fillet and pulp in the mixer, the temperature of the added fat, the temperature of water and the ratio of protein extraction time to emulsion time. The physicochemical analyses showed that the higher temperature of the pulp and fillet of fish, the lower the protein in the final product. Microbiological analyses revealed that the counting of Staphylococcus coagulase positive, total and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fish nuggets; Experimental design; Process parameters. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612011000200019 |
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| Registros recuperados: 13 | |
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