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Development of an Extraction Protocol for Fetal Sexing from the Fourth Week of Gestation by Real Time PCR BABT
Oliveira,Edwin José Torres de; Rabacow,Ana Paula Maluf; Figueiredo,Bruno Pereira de; Ishikawa,Raíssa Borges; Pessatto,Lucas Roberto; Pelizaro,Bruno Ivo; Figueiredo,Helder Pereira de; Oliveira,Rodrigo Juliano.
Abstract Low number of fetal cells in maternal blood limited the use of fetal materials in diagnostic and clinical applications. This research developed a technology which allowed the extraction of fetal DNA by a non-invasive method that offers no risk to the mother or fetus. A total of 132 pregnant women participated in this inquiry. The DNA extraction was performed employing an in-house method based on guanidine thiocyanate and magnetic bead. For the amplification it was used the Quantifier Y Kit™. The fetal sexing analysis of the 132 pregnant women were 100% in agreement with the ultrasound. Sensitivity and specificity for detection of Y chromosome sequences was possible using Real-Time Polymerase Chain Reaction since the 4th week of gestation. This...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fetal-sexing; DNA extraction; RtPCR..
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100416
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