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PCR-based identification of Neospora caninum in the umbilical cord of a newborn calf in Brazil Ciência Rural
Azevedo da Cunha Filho,Nilton; Oliveira,Plínio Aguiar de; Oliveira,Fernando Caetano de; Pappen,Felipe Geraldo; Aguiar,Cintia Lidiane Guidotti; Santos Junior,Alceu Gonçalves dos; Costa-da-Silva,André Luis; Leite,Fabio Pereira Leivas; Farias,Nara Amélia da Rosa.
ABSTRACT: This study was conducted at a beef cattle breeding farm in the far southern region of Brazil. The birth of a calf with unilateral corneal opacity was immediately reported to the Laboratory of Parasitology, in the Universidade Federal de Pelotas, Rio Grande do Sul, Brazil; blood was collected from the cow and calf before colostrum intake. The umbilical cords from this calf and from six other healthy animals were collected. Serological examination, utilizing an indirect fluorescent antibody test, was done using a cut-off point of 1:100. Polymerase chain reaction (PCR) was also performed using the umbilical cord samples. Serological tests showed that the calf was positive for Neospora caninum at birth, with a titer of 1:1600; a titer of 1:3200 was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Neospora caninum; Calves; Umbilical cord; Bovine.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000700501
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The incidence and productive significance of ovine toxoplasmosis in Southern Brazil Ciência Rural
Oliveira,Fernando Caetano de; Oliveira,Plínio Aguiar de; Cunha Filho,Nilton Azevedo da; Aguiar,Cintia Lidiane Guidotti; Pappen,Felipe Geraldo; Ruas,Jeronimo Lopes; Farias,Nara Amélia da Rosa.
ABSTRACT: This research aimed to determine incidence of Toxoplama gondii infection in Corriedale sheep in southern Brazil during pregnancy and the extent of associated losses. Blood samples were collected from 411 Corriedale sheep in two flocks at different locations in the state of Rio Grande do Sul (Brazil). Presence of T. gondii was diagnosed by the Indirect Fluorescent Antibody Technique (IFAT). Seroprevalence of T. gondii during pre-mating was 20.2%, with no significant difference between the two flocks studied. Infection by T. gondii was influenced by ewes'age (P≤0.05). The pre-mating seroprevalence did not influence either returning to estrus or pregnancy rates. Weaning rate was significantly higher in pre-positive dams compared to negative...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Toxoplasma gondii; Ewe; Pregnancy; Losses.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000901618
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Urolithiasis with uroperitoneum and hydronephrosis in grazing cattlein Southern Brazil Ciência Rural
Santos,Bianca Lemos dos; Pereira,Clairton Marcolongo; Coelho,Ana Carolina Barreto; Silva,Pablo Estima; Oliveira,Plínio Aguiar de; Stigger,Adriana Lücke; Schild,Ana Lucia.
ABSTRACT: The purpose of this paper was to describe obstructive urolithiasis with uroperitoneum and hydronephrosis in range cattle in southern Rio Grande do Sul. The disease was observed in castrated male cattle, aged between 18 months and 3 years in a herd of 200 steers that were in a ryegrass pasture during the winter and a native pasture during the summer. Different mineral salts were used as a supplement for the animals during the two seasons of the year. Cattle presented a progressive abdominal distention, depression and dehydration ranging from 10 days to one month in duration. One animal was necropsied, and approximately 50 liters of urine (uroperitoneum) in the abdominal cavity were observed. The bladder had a small rupture and was adhered to the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ruminants; Uroliths; Hydronephrosis.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000600554
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