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Oliveira,Gilmara Alvarenga Fachardo; Pádua,Juliano Gomes; Costa,Juliana Leles; Jesus,Onildo Nunes de; Carvalho,Fabiana Moraes de; Oliveira,Eder Jorge de. |
The aim of this study was to evaluate the selected 41 SSR markers developed for yellow passion fruit (Passiflora edulis f. flavicarpa Sims.) for their transferability to 11 different Passiflora species. Twenty-one SSR were successfully amplified in 10 wild species of passion fruit producing 101 bands. All the markers were amplifiable for at least one species. The mean transferability was 68,8%, ranging from 15,4% (primer PE11) to 100 % (PE13, PE18, PE37, PE41 and PE88). Transferability was higher for the species from the Passiflora subgenus than for those from the Decaloba and Dysosmia subgenus. The results indicated a high level of nucleotide sequence conservation of the primer regions in the species evaluated, and consequently, they could potentially be... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Transferability; SSR; Passion fruit. |
Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500009 |
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Oliveira,Eder Jorge; Pádua,Juliano Gomes; Zucchi,Maria Imaculada; Vencovsky,Roland; Vieira,Maria Lúcia Carneiro. |
Microsatellites, or simple sequence repeats (SSRs), have been the most widely applied class of molecular markers used in genetic studies, with applications in many fields of genetics including genetic conservation, population genetics, molecular breeding, and paternity testing. This range of applications is due to the fact that microsatellite markers are co-dominant and multi-allelic, are highly reproducible, have high-resolution and are based on the polymerase chain reaction (PCR). When first introduced, the development of microsatellite markers was expensive but now new and efficient methods of repetitive sequence isolation have been reported, which have led to reduced costs and microsatellite-technology has been increasingly applied to several species,... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Microsatellites; Molecular genetics; Genetic structure of populations. |
Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200018 |
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Santos,Carlos Antonio Fernandes; Castro,José Mauro da Cunha e; Souza,Flavio de França; Vilarinho,Aloísio Alcântara; Ferreira,Francisco Ricardo; Pádua,Juliano Gomes; Borges,Rita Mércia Estigarribia; Barbieri,Rosa Lia; Souza,Aparecida das Graças Claret de; Rodrigues,Marciene Amorim. |
The objective of this work was to characterize 119 accessions of guava and 40 accessions of "araçá" sampled in 35 Brazilian ecoregions, according to the International Union for the Protection of New Varieties of Plants (UPOV) descriptors. The majority of "araçá" accessions presented wide spacing of leaf veins, while guava accessions presented medium to close spacing. Most fruits of "araçá" accessions were classified as small, contrasting with medium to large fruits of guava accessions. Most of "araçá" accessions (91%) presented white flesh fruit color, while 58% of guava accessions presented pale pink, pink and dark pink colors. Fruit differences among wild and cultivated Psidium species indicate fruit as the most altered trait under artificial selection. |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Psidium guajava; Araçá; Guava. |
Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000300020 |
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José,Solange Carvalho Barrios Roveri; Salomão,Antonieta Nassif; Mundim,Rosângela Caldas; Pádua,Juliano Gomes. |
Dentre os processos que precedem a conservação em longo prazo, a secagem tem papel fundamental, uma vez que o conteúdo de água das sementes afeta diretamente a sua longevidade. Os objetivos desse trabalho foram pesquisar o efeito da umidificação prévia das sementes após ultrassecagem em diferentes teores de água sobre a qualidade fisiológica de sementes de girassol. Sementes com conteúdo inicial de água de 4,7% foram embebidas previamente até conteúdo de água de 10,2%, e submetidas à secagem lenta, conduzida em câmara de secagem, e à secagem rápida, em sílica gel, até conteúdos de água de 7,4; 3,2; 2,9% e 5,3; 3,2; 2,1%, respectivamente. Após a secagem rápida ou lenta, as sementes foram submetidas ou não à umidificação em caixas tipo "gerbox" até o teor de... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dessecação; Helianthus annuus; Germinação; Vigor. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-31222009000300002 |
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