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	Provedor de dados BJM (1) Autor Padmanabhan,Sriram (1) Rao,Sneha (1) Sundarrajan,Sudarson (1) Palavra-chave DNA virus (1) Hepatitis B virus (1) RecA (1) Thermus thermophilus (1) Tipo do documento Info:eu-repo/semantics/article (1) Ano 2018 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Cloning and high-level expression of Thermus thermophilus RecA in E. coli: purification and novel use in HBV diagnostics BJM Sundarrajan,Sudarson; Rao,Sneha; Padmanabhan,Sriram. ABSTRACT We studied the role of Thermus thermophilus Recombinase A (RecA) in enhancing the PCR signals of DNA viruses such as Hepatitis B virus (HBV). The RecA gene of a thermophilic eubacterial strain, T. thermophilus, was cloned and hyperexpressed in Escherichia coli. The recombinant RecA protein was purified using a single heat treatment step without the use of any chromatography steps, and the purified protein (>95%) was found to be active. The purified RecA could enhance the polymerase chain reaction (PCR) signals of HBV and improve the detection limit of the HBV diagnosis by real time PCR. The yield of recombinant RecA was ∼35 mg/L, the highest yield reported for a recombinant RecA to date. RecA can be successfully employed to enhance detection... Tipo: Info:eu-repo/semantics/article Palavras-chave: RecA; Hepatitis B virus; DNA virus; Thermus thermophilus. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400848 Registros recuperados: 1 Primeira ... 1 ... Última

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