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| Prudente, Débora de Oliveira; Nery, Fernanda Carlota; Paiva, Renato; Goulart, Vívian Loryane Ávila; Anselmo, Aline Cristini do Nascimento. |
| Candeia (Eremanthus elytropappus) is a native species from Brazilian Cerrado and presenting economic potential for the timber and pharmaceutical industry. However, the ex vitro germination rate of the seeds is considered low. In this context, the objective was to establish a protocol for in vitro germination and multiplication, aiming at the rapid multiplication of the species. For the in vitro germination medium were tested WPM; ½ WPM; ¼ WPM; MS; MS ½ and ¼ MS. In addition, Were Also tested GA3 (0.0; 0.2; 0.4; 0.6; 0.8 mg L-1) and pH levels (4.8, 5.8 and 6.8) in ¼ WPM culture medium. For in vitro multiplication, stem segments were inoculated in ¼ WPM supplemented with BAP (0.0, 1.0, 2.0, 3.0, 4.0 and 5.0 mg L-1). Shoots obtained in vitro were individually... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cerrado; Cultura de tecidos; Eremanthus erythropappus (DC.) MacLeish; Espécie florestal.. |
| Ano: 2016 |
URL: http://e-revista.unioeste.br/index.php/scientiaagraria/article/view/12893 |
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| Prudente, Débora de Oliveira; Nery, Fernanda Carlota; Silva, Laís Silveira da; Paiva, Renato; Reis, Michele Valquíria dos; Nery, Marcela Carlota. |
| The paineira-rosa (Chorisia speciosa St. Hil) is an arboreal species of great importance in restoring degraded ecosystems and riparian forests, however, the availability of seeds of this species is low. The objective was to establish in vitro germination protocols and cryopreservation of paineira-rosa seeds. For the in vitro germination, MS culture medium and WPM was tested three concentrations of sucrose (0, 15, 30 g L-1) and three pH levels (4.8, 5.8 and 6.8). For cryopreservation, the seeds were dried on silica gel or laminar flow for different times (0, 1, 2, 3 and 4 h) and then were stored in liquid nitrogen (-196 °C) for 24 h, defrosted in a water bath (38 °C) for four minutes and they were inserted into test tubes containing WPM medium cultivation.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Armazenamento em longo prazo; Chorisia speciosa; Dessecação; Espécie florestal.. |
| Ano: 2016 |
URL: http://e-revista.unioeste.br/index.php/scientiaagraria/article/view/11775 |
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| Santos, Paulo Augusto Almeida; Paiva, Renato; Silva, Luciano Coutinho; Souza, Ana Cristina de; Santana, Marlucia Cruz de; Silva, Diogo Pedrosa Corrêa da. |
| The aim of this study was to evaluate the efficiency of vitrification and droplet vitrification for the cryopreservation of Hancornia speciosa shoot tips. The shoot tips were subjected to four different periods of exposure (15, 30, 45 and 60 min.) to plant vitrification solution 2 (PVS2) before plunging into liquid nitrogen. We evaluated the regrowth of H. speciosa shoot tips that were cryopreserved by the classical vitrification technique and by the droplet vitrification technique. Shoot tips were submitted to different periods of pre-culture (absence, 24 or 48h) in a medium containing 0.3 M sucrose prior to cryopreservation. With a PVS2 exposure period of 60 min., significant differences were observed between the two techniques used in this study with... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: 5.00.00.00-4 - ciências agrárias; 2.03.03.00-9 - fisiologia vegetal; Native species; In vitro conservation; Recalcitrant seeds; Plant genetic diversity conservation; Vitrification; Droplet vitrification Conservação in vitro; Criopreservação vegetal. |
| Ano: 2015 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/19573 |
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