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| Papamichael,Emmanuel M.; Evmiridis,Nickolaos P.; Potosis,Constaninos. |
| In this work, we present a non-parametric method, and the appropriate computer program, for fitting nonlinear multiparametric equations to experimental data. Our method is followed by computation of confidence limits of the parameter estimates. Its performance has been tested on several multiparametric equations, common in the fields of Biochemistry and Biotechnology, and it is a multiparametric expansion of the concept proposed by others for equations having more than two parameters. Good parameter estimates were obtained without a previous knowledge of initial parameter guessing values, and the proposed computer program converges rapidly, in all cases examined within this work. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Non-parametric methods; Computer program; Fitting of nonlinear equations. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000100001 |
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| Kokkinou,Marina; Theodorou,Leonidas G.; Papamichael,Emmanuel M.. |
| This article reports a first contribution for the elucidation of catalytic mechanism of Lipase from porcine pancreas, type VI-s (PPL), in hydrolyzing an ester substrate in aqueous media. The conclusions were based on the pH-profiles of Michaelis-Menten parameters k cat/Km, k cat and Km, as well as on the absolute temperature profile of k cat/Km, obtained during the hydrolysis of p-nitrophenyl laurate by PPL. It was found that (a) PPL performs catalysis by means of ion pairs formed either as Ser152-Ο-/His263-Im+H and/or Carbonyl-Ο-/His263-Im+H, (b) the parameter k cat/Km equals to k1 and thus ES is formed and destroyed in the course of a series of consecutive reactions governed by the dynamic constant K S = k2/k1, and (c) the hydrolysis of substrate is... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Porcine Pancreas Lipase; Mechanism of hydrolysis; P-nitrophenyl laurate. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000200007 |
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