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	Provedor de dados BJM (2) BABT (1) Autor Paschoalin,Vânia Margaret Flosi (3) Silva,Joab Trajano (3) Figueiredo,Eduardo Eustáquio de Souza (2) Fonseca,Leila Sousa (2) Lilenbaum,Walter (2) Silvestre,Flávia Galindo (2) Campos,Wilma Neres (1) Carvalho,Ricardo Cezar Tavares (1) Conte-Junior,Carlos Adam (1) Furlanetto,Leone Vinícius (1) Golinelli,Luciana Pacheco (1) Medeiros,Luciana (1) Mais... Palavra-chave Bovine tuberculosis (2) Multiplex-PCR (2) Mycobacterium bovis (2) Bovine whey proteins (1) Colostrum proteins (1) Low-abundance proteins (1) Mass spectrometry (1) Matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI TOF/TOF MS) (1) Nasal swab (1) Two-dimensional electrophoresis (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Ano 2011 (1) 2010 (1) 2009 (1) País Brazil (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Proteomic analysis of whey from bovine colostrum and mature milk BABT Golinelli,Luciana Pacheco; Conte-Junior,Carlos Adam; Paschoalin,Vânia Margaret Flosi; Silva,Joab Trajano. The aim of this study was to standardize a methodology to obtain two-dimensional (2D) maps of whey proteins from bovine colostrum and mature milk, using two-dimensional electrophoresis, in order to identify the minor proteins by matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI TOF/TOF MS). A total of 38 proteins were identified, 20 spots in the colostrum whey and 18 in the mature milk whey; 5 of them were identified for the first time. Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine whey proteins; Colostrum proteins; Low-abundance proteins; Two-dimensional electrophoresis; Mass spectrometry; Matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI TOF/TOF MS). Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400016 Identification of Mycobacterium bovis Isolates by a multiplex PCR BJM Figueiredo,Eduardo Eustáquio de Souza; Silvestre,Flávia Galindo; Campos,Wilma Neres; Furlanetto,Leone Vinícius; Medeiros,Luciana; Lilenbaum,Walter; Fonseca,Leila Sousa; Silva,Joab Trajano; Paschoalin,Vânia Margaret Flosi. Isolates from suggestive bovine tuberculosis lesions were tested by a multiplex polymerase chain reaction (m-PCR) targeting for RvD1Rv2031c and IS6110 sequences, specific for M. bovis and Mycobacterium tuberculosis complex respectively. The m-PCR successfully identified as M. bovis 88.24% of the isolates. Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycobacterium bovis; Multiplex-PCR; Bovine tuberculosis. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200004 Detection of Mycobacterium bovis DNA in nasal swabs from tuberculous cattle by a multiplex PCR BJM Figueiredo,Eduardo Eustáquio de Souza; Carvalho,Ricardo Cezar Tavares; Silvestre,Flávia Galindo; Lilenbaum,Walter; Fonseca,Leila Sousa; Silva,Joab Trajano; Paschoalin,Vânia Margaret Flosi. Detection of tuberculosis in cattle relies on the intradermal tuberculin test (ITT), but a definitive diagnosis requires identification of the pathogen after the animal is slaughtered. DNA in nasal swabs from 50 cows was analyzed by m-PCR, targeting for the RvD1-Rv2031c and IS6110 sequences. M. bovis was identified in two of 34 tuberculous cows (5.9%). The use of mPCR of nasal swabs as an in vivo diagnostic tool for bovine tuberculosis is suggested. Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycobacterium bovis; Multiplex-PCR; Nasal swab; Bovine tuberculosis. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200020 Registros recuperados: 3 Primeira ... 1 ... Última

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