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Statistical investigation of Kluyveromyces lactis cells permeabilization with ethanol by response surface methodology BJM
Faria,Janaína T. de; Rocha,Pollyana F.; Converti,Attilio; Passos,Flávia M.L.; Minim,Luis A.; Sampaio,Fábio C..
The aim of our study was to select the optimal operating conditions to permeabilize Kluyveromyces lactis cells using ethanol as a solvent as an alternative to cell disruption and extraction. Cell permeabilization was carried out by a non-mechanical method consisting of chemical treatment with ethanol, and the results were expressed as β-galactosidase activity. Experiments were conducted under different conditions of ethanol concentration, treatment time and temperature according to a central composite rotatable design (CCRD), and the collected results were then worked out by response surface methodology (RSM). Cell permeabilization was improved by an increase in ethanol concentration and simultaneous decreases in the incubation temperature and treatment...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Yeast; Cheese whey; Permeabilization; Ethanol; Β-galactosidase.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400007
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