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	Provedor de dados BJM (3) Rev. Microbiol. (1) Ciência e Agrotecnologia (1) Electron. J. Biotechnol. (1) Autor Pastore,Gláucia Maria (6) Alves Macedo,Gabriela (1) Bicas,Juliano Lemos (1) Carvalho,Patrícia de Oliveira (1) Cruz,Juliana Aparecida Bortollotti (1) Duarte,Marta Cristina Teixeira (1) Koblitz,Maria Gabriela Bello (1) Oliveira,Joaquim Gilberto de (1) Prazeres,Janaina Nicanuzia dos (1) Quadros,Cedenir Pereira de (1) Soberón Lozano,Maria Mercedez (1) Mais... Palavra-chave Alkaline lipase (1) Antimicrobial activity (1) Biochemical characterization (1) Biotechnology (1) Detergents (1) Flavor (1) Fusarium oxysporum (1) Gamma-linolenic acid (1) Industrial by-products (1) Limonene (1) Lipase (1) Listeria innocua (1) Microbial biofilm (1) Minimum inhibitory concentration (1) Mucor sp (1) Purification (1) Rhizopus sp. (1) Stainless steel surface (1) Surfactants (1) Unsaturated fatty acids (1) Mais... Tipo do documento Info:eu-repo/semantics/article (5) Journal article (1) Ano 2011 (1) 2007 (1) 2006 (2) 2003 (1) 1999 (1) País Brazil (5) Chile (1) Idioma Inglês (6)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 6 Primeira ... 1 ... Última Enzymatic synthesis of short chain citronellyl esters by a new lipase from Rhizopus sp Electron. J. Biotechnol. Alves Macedo,Gabriela; Soberón Lozano,Maria Mercedez; Pastore,Gláucia Maria. Short chain citronellyl esters were synthesized by a new microbial lipase from Rhizopus sp strain isolated and lipase produced at UNICAMP, Brazil. Direct esterification and transesterification reactions have been performed to produce citronellyl acetate and butyrate in a free-solvent system and with n-hexane in reaction medium. Reaction mixture for direct esterification and transesterification was carried out at 45ºC in equimolar concentration of substrates: acid or ester and alcohol. Only citronellyl butyrate was synthesized by direct esterification with yields from 95 to 100% after 24 hrs. of reaction time, with or without n-hexane. Citronellyl acetate was synthesized by transesterification with ethyl acetate and cytronellol, with yield of 58% after 48... Tipo: Journal article Ano: 2003 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582003000100009 Enhancement of gamma-linolenic acid production by the fungus Mucor sp LB-54 by growth temperature Rev. Microbiol. Carvalho,Patrícia de Oliveira; Oliveira,Joaquim Gilberto de; Pastore,Gláucia Maria. As a relatively prolific producer of GLA, the strain of Mucor sp LB-54 was selected for a study at different growth temperatures in shaker flask culture. The strain used in our experiment was capable to accumulate a relatively high amount of intracellular lipid, 20.73 % of dry cell weight, and GLA content of 15 % of total fatty acids after 5 days of incubation at 28°C. As the growth temperature was decreased from 28 to 12°C the percentage of GLA increased from 15 to 24 % of total fatty acids. In order to optimize the culture conditions for rapid biomass production and lipid production with a high proportion of GLA, the fungus was grown at two temperature combinations associated supplies of carbon source (glucose) in the culture medium. Maximal production... Tipo: Info:eu-repo/semantics/article Palavras-chave: Gamma-linolenic acid; Mucor sp; Unsaturated fatty acids. Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000200016 Characterization of alkaline lipase from Fusarium oxysporum and the effect of different surfactants and detergents on the enzyme activity BJM Prazeres,Janaina Nicanuzia dos; Cruz,Juliana Aparecida Bortollotti; Pastore,Gláucia Maria. Nowadays, there is a tendency of use of low temperature in laundry cleaning for both environmental and economical reasons, which makes the use of enzymes in detergent products indispensable. Since lipases are efficient catalyst both in solution and at the water-liquid interface, they are potentially suitable for lipid stain removal applications in industrial laundry and household detergents. The effect of different commercial detergents and surfactants on enzymatic activity of lipase from Fusarium oxysporum was observed through p-nitrophenylpalmitate (pNPP) assay. The enzyme was compatible with various ionic and non-ionic surfactants as well as commercial detergents. Lipase activity was strongly inhibited by Sodium Dodecyl Sulphate (SDS), but not by Triton... Tipo: Info:eu-repo/semantics/article Palavras-chave: Alkaline lipase; Fusarium oxysporum; Surfactants; Detergents. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400019 Isolation and screening of d-limonene-resistant microorganisms BJM Bicas,Juliano Lemos; Pastore,Gláucia Maria. This study reports the isolation of microorganisms that are resistant to environment containing limonene, the most important residue in the citrus industry. For the isolation, samples collected from strategic places of a citrus processing plant (yellow water, entrance and exit of the bagasse tank, effluent and deteriorated fruits found in bins, machine straps, fruit washers and plant floor), some citrus fruit and mint from local market were used. The samples were incubated in rotary shaker at 30ºC/150rpm for 48h or 7 days in YM medium containing 0.1% limonene. Great part of the 112 strains recovered after 48h and the 126 strains recovered after 7 days were identified as Gram positive bacilli, followed by Gram negative bacilli, yeasts and Gram positive... Tipo: Info:eu-repo/semantics/article Palavras-chave: Limonene; Industrial by-products; Flavor; Biotechnology. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300034 Biological activities of a mixture of biosurfactant from Bacillus subtilis and alkaline lipase from Fusarium oxysporum BJM Quadros,Cedenir Pereira de; Duarte,Marta Cristina Teixeira; Pastore,Gláucia Maria. In this study, we investigate the antimicrobial effects of a mixture of a biosurfactant from Bacillus subtilis and an alkaline lipase from Fusarium oxysporum (AL/BS mix) on several types of microorganisms, as well as their abilities to remove Listeria innocua ATCC 33093 biofilm from stainless steel coupons. The AL/BS mix had a surface tension of around 30 mN.m-1, indicating that the presence of alkaline lipase did not interfere in the surface activity properties of the tensoactive component. The antimicrobial activity of the AL/BS mix was determined by minimum inhibitory concentration (MIC) micro-assays. Among all the tested organisms, the presence of the mixture only affected the growth of B. subtilis CCT 2576, B. cereus ATCC 10876 and L. innocua. The... Tipo: Info:eu-repo/semantics/article Palavras-chave: Antimicrobial activity; Listeria innocua; Microbial biofilm; Minimum inhibitory concentration; Stainless steel surface. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100045 Purification and biochemical characterization of an extracellular lipase produced by a new strain of Rhizopus sp. Ciência e Agrotecnologia Koblitz,Maria Gabriela Bello; Pastore,Gláucia Maria. The present study had as a goal to purify and characterize the lipolytic fraction secreted by a strain of Rhizopus sp. Only 3 steps of purification were necessary to achieve SDS-PAGE homogeneity. One band with 37.5 KDa molecular mass and with 1446 U/mg specific activity was obtained. The purified fraction presented 2 lipase isoforms; both showed optimum activity at 50ºC, and were stable between 6.5 and 7.5 pH values and at temperatures below 50ºC and also kept their activity in hexane. The lipase was inactivated by Hg+2 and by n-bromosuccinimide and activated by Na+. Tipo: Info:eu-repo/semantics/article Palavras-chave: Lipase; Rhizopus sp.; Purification; Biochemical characterization. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542006000300016 Registros recuperados: 6 Primeira ... 1 ... Última

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