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Histopathologic and mycologic aspects of experimental infection of guinea pigs with Microsporum canis Braz. J. Vet. Res. Anim. Sci.
Cavalcanti,Josemara Neves; Guerra,José Luiz; Gambale,Walderez; Corrêa,Benedito; Paula,Claudete Rodrigues.
Dermatophytosis is a zoonosis in whose etiology the dermatophyte Microsporum canis is frequently involved. The fungus can be transmitted to man by dogs and cats. In the present study, guinea pigs were experimentally inoculated with M. canis and the course of the lesions was evaluated. Inoculation resulted in lesions in 100% of the animals, with a clinical course consisting of an incubation period, an inflammatory and a phase of lesion resolution. The histopathologic evaluation of the skin biopsies revealed the presence of acanthosis, hyperkeratosis, spongiosis and marked dermal edema. M. canis spores and hyphae were detected in histologic sections stained with periodic acid-Schiff. The combination of the fluorescent dyes FD and EB permitted the adequate...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dermatophytosis; Microsporum canis; Ringworm; Guinea pigs.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962002000500004
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EXPERIMENTAL MODEL OF CANDIDA ALBICANS (SEROTYPES A AND B) ADHERENCE IN VITRO BJM
Pires,Maria de Fátima Costa; Corrêa,Benedito; Gambale,Walderez; Paula,Claudete Rodrigues.
The adherence of Candida albicans is considered an important virulence factor since it determines the colonization of the yeast in various regions of the host, being the initial cause of the infection. In this work we studied the adherence of C. albicans (serotypes A and B) to HeLa and Vero cells after culture for 18 h and 30 h in chemically defined medium (YNB) plus carbohydrate sources such as glucose, sucrose, maltose, galactose and mannose (500 mM). Each assay was performed in triplicate. The best results were obtained with the medium containing glucose. The adherence was greatest in 18 h cultures at 37ºC and at 1 h contact time. The interaction of yeast cells with the host cells was studied by scanning microscopy.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Candida albicans; Adherence; Serotypes.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000300001
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