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| Fernandes,A.M.; Marinho,P.A.N.; Sartore,R.C.; Paulsen,B.S.; Mariante,R.M.; Castilho,L.R.; Rehen,S.K.. |
| Future clinical applications of human embryonic stem (hES) cells will require high-yield culture protocols. Currently, hES cells are mainly cultured in static tissue plates, which offer a limited surface and require repeated sub-culturing. Here we describe a stirred system with commercial dextran-based microcarriers coated with denatured collagen to scale-up hES cell production. Maintenance of pluripotency in the microcarrier-based stirred system was shown by immunocytochemical and flow cytometry analyses for pluripotency-associated markers. The formation of cavitated embryoid bodies expressing markers of endoderm, ectoderm and mesoderm was further evidence of maintenance of differentiation capability. Cell yield per volume of medium spent was more than... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Human embryonic stem cells; Scale-up; Microcarriers; Spinner bioreactors. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000600007 |
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