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| Prince,Karina Andrade de; Sordi,Renata; Pavan,Fernando Rogério; Santos,Adolfo Carlos Barreto; Araujo,Angela R.; Leite,Sergio R.A.; Leite,Clarice Q. F.. |
| Our purpose was to determine the anti-Mycobacterium tuberculosis activity of the metabolites produced by the endophitic fungus Phomopsis stipata (Lib.) B. Sutton, (Diaporthaceae), cultivated in different media. The antimycobacterial activity was assessed through the Resazurin Microtiter Assay (REMA) and the cytotoxicity test performed on macrophage cell line. The extracts derived from fungi grown on Corn Medium and Potato Dextrose Broth presented the smallest values of Minimum Inhibitory Concentration (MIC) and low cytotoxicity, which implies a high selectivity index. This is the first report on the chemical composition and antitubercular activity of metabolites of P. stipata, as well as the influence of culture medium on these properties. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Phomopsis stipata; Antimycobacterial activity; Mycobacterium tuberculosis. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100024 |
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| Miyata,Marcelo; Pavan,Fernando Rogério; Sato,Daisy Nakamura; Marino,Leonardo Biancolino; Hirata,Mario Hiroyuki; Cardoso,Rosilene Fressati; Melo,Fernando Augusto Fiúza de; Leite,Clarice Queico Fujimura. |
| We assessed the performance of REMA in comparison with BACTEC MGIT 960 in the susceptibility testing of 80 Mycobacterium tuberculosis clinical isolates from Clemente Ferreira Institute against four drugs. REMA proved to be a rapid and accurate method, providing excellent correlation with BACTEC MGIT 960, with the exception of results for the ethambutol drug. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mycobacterium tuberculosis; Tuberculosis; Drug susceptibility testing. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100041 |
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| Cardoso,Claudia Andréa Lima; Coelho,Roberta Gomes; Honda,Neli Kika; Pott,Arnildo; Pavan,Fernando Rogério; Leite,Clarice Queico Fujimura. |
| Serjania erecta Radlk.(Sapindaceae) is a medicinal plant traditionally used in Brazil. We assayed the ethanolic extract of leaves and roots against seven microorganisms. The REMA (Resazurin Microtiter Assay) assay was used to measure the biological activity in vitro against Mycobacterium tuberculosis and our results showed moderate activity of the ethanolic extract. On the other hand S. aureus, P. aeruginosa, S. setubal, C. albicans, S. cerevisiae and E. coli revealed that the leaves and roots of S. erecta inhibited the growth of all microorganisms. The ethanolic extracts of leaves and roots showed low values of antioxidant activities. The ethanolic extracts of leaves and roots were analyzed by chromatographic and spectrometric methods. (-)-Epicatechin,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Serjania erecta Radlk/pharmacognosy; Serjania erecta Radlk/ethanolic extract; Serjania erecta Radlk/antioxidant activity; Serjania erecta Radlk/ethanolic extract; Serjania erecta Radlk/antimicrobial activity; Sapindaceae/pharmacognosy; Cipó-cinco-folhas/pharmacognosy; Flavonoids; Medicinal plants. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000400017 |
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| Amaral,Renata Claro Ribeiro do; Caleffi-Ferracioli,Katiany Rizzieri; Demitto,Fernanda de Oliveira; Almeida,Aryadne Larissa de; Siqueira,Vera Lucia Dias; Scodro,Regiane Bertin de Lima; Leite,Clarice Queico Fujimura; Pavan,Fernando Rogério; Cardoso,Rosilene Fressatti. |
| The membrane-based efflux pump systems are recognized to have an important role in pathogenicity and drug resistance in Mycobacterium tuberculosis by the extrusion of toxic substrates and drugs from the inner bacillus. This study aimed to investigate the in vitro interaction of Verapamil (VP), an efflux pump inhibitor, with the classical first-line anti-tuberculosis drug isoniazid (INH) in resistant and susceptible M. tuberculosis clinical isolates. Seven multidrug-resistant (MDR), three INH monoresistant and four susceptible M. tuberculosis clinical isolates were tested for the INH and VP combination by modified Resazurin Microtiter Assay Plate (REMA). Fractional Inhibitory Concentration (FIC) and Modulation Factor (MF) were determined. The INH plus VP... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Tuberculosis; Multidrug-resistance; Efflux pumps; Efflux pumps inhibitors; Isoniazid. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100588 |
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| Caleffi-Ferracioli,Katiany Rizzieri; Baldin,Vanessa Pietrowski; Noguti,Érika Noda; Siqueira,Vera Lucia Dias; Scodro,Regiane Bertin Lima; Pavan,Fernando Rogério; Hirata,Mario Hiroyuki; Cardoso,Rosilene Fressatti. |
| Tuberculosis (TB) is an infectious disease in which the molecular typing methods allow to have important information about the dynamics of transmission and to assist properly in disease control. Although the ERIC-PCR (Enterobacterial repetitive intergenic consensus-PCR) assay is fast and easy to perform, scarce studies have reported its use in epidemiological studies in TB outbreaks. In this study, we aimed to genotype Mycobacterium tuberculosis and M. bovis isolates by ERIC-PCR and compare its discriminatory power with two other classically used methods: 12 loci-MIRU (Mycobacterial Interspersed Repetitive Units) and Spoligotyping. The M. tuberculosis isolates studied were from northwestern and southwestern and M. bovis from northwestern Parana, Brazil.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Tuberculosis; Molecular characterization; ERIC-PCR; Epidemiology. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000300616 |
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| Gatti,Tiago Henrique Honorato; Eloy,Josimar Oliveira; Ferreira,Leonardo Miziara Barboza; Silva,Isabel Cristine da; Pavan,Fernando Rogério; Gremião,Maria Palmira Daflon; Chorilli,Marlus. |
| Abstract Mucoadhesive nanoparticles are particularly interesting for delivery through nasal or pulmonary routes, as an approach to overcome the mucociliary clearance. Moreover, these nanoparticles are attractive for peptide and protein delivery, particularly for insulin to treat diabetes, as an alternative to conventional parenteral administration. Thus, chitosan, a cationic mucoadhesive polysaccharide found in shells of crustaceans, and the negatively-charged dextran sulfate are able to form nanoparticles through ionic condensation, representing a potential insulin carrier. Herein, chitosan/dextran sulfate nanoparticles at various ratios were prepared for insulin loading. Formulations were characterized for particle size, zeta potential, encapsulation... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Insulin; Chitosan; Dextran-Sulfate; Nanoparticles; Mucoadhesion.. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000100623 |
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