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Toxin content of Ostreopsis cf. ovata depends on bloom phases, depth and macroalgal substrate in the NW Mediterranean Sea ArchiMer
Gémin, Marin-pierre; Réveillon, Damien; Hervé, Fabienne; Pavaux, Anne-sophie; Tharaud, Maxime; Séchet, Veronique; Bertrand, Samuel; Lemée, Rodolphe; Amzil, Zouher.
Over the last fifteen years, blooms of the genus Ostreopsis have been reported more frequently and at higher abundances in the Mediterranean area. Ostreopsis cf. ovata is known to produce ovatoxins (OVTXs), structural analogues of palytoxin, which is one of the most potent non-polymeric toxins. However, the production of OVTXs is poorly characterized in situ. The present study focuses on toxin content and profile according to the bloom phase during summer 2017 in Villefranche-sur-Mer, France (NW Mediterranean Sea), depth (from 0.5 to 5 m) and three different macroalgal substrates of this epiphytic dinoflagellate (Padina pavonica, Dictyota spp. and Halopteris scoparia). Ovatoxin quantification of all samples was performed by liquid chromatography coupled to...
Tipo: Text Palavras-chave: Dinoflagellate; Benthic HABs; Ostreopsis cf. ovata; Ovatoxins; Mediterranean Sea.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00614/72566/74835.pdf
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Efficient, fast and inexpensive bioassay to monitor benthic microalgae toxicity: Application to Ostreopsis species ArchiMer
Pavaux, Anne-sophie; Ternon, Eva; Dufour, Louison; Marro, Sophie; Gémin, Marin-pierre; Thomas, Olivier P.; Lemée, Rodolphe.
Even though HPLC-MS is commonly used to quantify the toxin content of Ostreopsis spp. cells, there is a need to develop easy-to-use toxicological tests to set thresholds during Ostreopsis spp. blooms. The crustacean Artemia has been widely used to evaluate the presence and toxicity of chemicals and biological contaminants and we anticipated that it could also be useful to test Ostreopsis spp. toxicity. Its relevance was first assessed by investigating the variability of the toxic effects among Ostreopsis spp. strains and throughout the dinoflagellate life cycle in combination with chemical analyses of the toxinic content by UHPLC-HRMS. After testing the toxicity of fractions prepared from Ostreopsis spp. cells, the known ova- and paly-toxins were not the...
Tipo: Text Palavras-chave: Ostreopsis spp.; Anemia franciscana; Bioassay; Ecotoxicity; HPLC-HRMS.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00624/73625/73051.pdf
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