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การจัดทำลายพิมพ์เอกลักษณ์ดีเอ็นเอข้าวพื้นเมืองไทยที่เก็บอยู่ในธนาคารเชื้อพันธุ์ข้าวแห่งชาติ 106
Payorm Cobelli; Varapong Chamarerk; Poonsak Mekwatanakarn; Phikul Leelagud; Somsong Chotechuen; Sommai Seewisut.
SSR molecular markers SSR are tandem arranged repeats of short DNA motifs (1-6 bp in length) distributed throughout the genome that frequently exhibit variation in the number of repeats at the locus of each rice variety. The SSR markers, therefore, are suitable for detecting polymorphisms between closely related genotypes of Thai local rice varieties from the National Rice Germplasm Bank. In this research, DNA fingerprinting was conducted using 8 Thai local rice varieties. i.e., Niaw Sanpahtawng (NSPT_GB), Khao Gaw Diaw (KGD_GB), Niaw Dam (ND_GB), Khao tah Haeng 17 (KTH17_GB), Jao’ Haw (JH_GB), Pin Gaew 56 (PG56_GB), Jek Chuey Gahb Khiaw (JCGK_GB), Sang Yod Pattalung (SYPL_GB), and 5 standard varieties, i.e., Khao Dawk Mali 105 (KDML105_GB, KDML105_C),...
Tipo: PhysicalObject Palavras-chave: Thai local rice; DNA fingerprinting; Molecular markers; National Rice Germplasm Bank; ข้าวพื้นเมืองไทย; ลายพิมพ์เอกลักษณ์ดีเอ็นเอ; เครื่องหมายโมเลกุล; การเรียงตัวของดีเอ็นเอ; ความใกล้ชิดทางพันธุกรรม; พันธุ์.
Ano: 2012 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5225
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การตรวจวิเคราะห์สินค้าข้าวดัดแปรงพันธุกรรมด้วยปฏิกิริยาลูกโซ่ในสภาพเวลาจริง 106
Varapong Chamarerk; Roongnapa Kawichai; Payorm Cobelli; Poonsak Mekwatanakarn.
Currently, there are several reports on GM rice illegally available on market. To set up a defense measure in order to prevent GM contamination in Thai rice market, this activity was conducted by analyzing for CaMV 35S Promoter, NOS terminator and a transgene CryIA(b), which were carrying by the Bt63 rice. The Bt63 rice is an insect-resistant rice variety developed by the People Republic of China. The analyses were done by using a Real-Time PCR technique. DNA samples were extracted from rice products using a GeneScan extraction method and confirmed by a PCR using a primer pair KVM159/KVM160, which amplifies a rice species specific PLD gene encoding for an enzyme Phospholipase D. It was found that all samples could be amplified showing a PLD gene with a...
Tipo: Collection Palavras-chave: GM rice; Bt63 rice; Real-time PCR; ข้าวดัดแปรพันธุกรรม; ข้าว Bt63; ปฏิกิริยาลูกโซ่แบบในสภาพเวลาจริง.
Ano: 2015 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5850
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การตรวจสอบความหลากหลายของสายพันธุ์เชื้อราสาเหตุโรคไหม้ของข้าวในประเทศไทย 106
Poonsak Mekwatanakarn; Payorm Cobelli; Acharaporn Na Lampang Noenplab; Thanomjit Rithmontree; Kulchana Ketsuwan; Chanasirin Klinmanee; Sanguan Teang Deerith.
Rice blast disease is the major disease in rainfed lowland area of Thailand. The causal fungus of this disease is Pyricularia grisea Sacc. Variation of this fungus was higher than other rice pathogens. This report used near isogenic lines (NILs) to detect the variation, pathothype and virulence spectrum of the fungus in Thailand during 2002 - 2005. Trap blast nurseries consisted of 9 Thai local cultivars and 18 NILs were conducted at Ubon Ratchathani Rice Research Center, Khon Kaen Rice Research Center, Phrae Rice Research Center, Phitsanulok Rice Research Center, and Phatthalung Rice Research Center. Single conidia was isolated from infected rice leaves. Virulence spectrum of blast populations were screened on 18 NILs. Two thousand four hundred seventy...
Tipo: Collection Palavras-chave: Rice; Blast disease; Pathotype; Near isogenic lines; Diversity; ข้าว; โรคไหม้; เชื้อสาเหตุ; สายพันธุ์เชื้อ; ยีนต้านทานเดี่ยว; ความหลากหลายของเชื้อ; เชื้อรา.
Ano: 2007 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5300
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การใช้เครื่องหมายโมเลกุลชนิด STSs สำรวจแหล่งพันธุกรรมยีนต้านทาน 106
Payorm Cobelli; Jittima Wongnongwa; Varapong Chamarerk; Somsong Chotechuen; Rasamee Dhitikiattipong; Witchuda Rattanakarn; Nuchakarn Sinprasit; Teerada Wangsomboondee.
Bacterial blight (BB) disease of rice, caused by Xanthomonas oryzae pv. oryzae (Xoo), is one of the most serious diseases in all major rice-growing areas of Thailand. The most effective approach to control BB is the use of resistant varieties. However, the selection pressure from the usage of resistant cultivars may result in mutation of the pathogen population to overcome the resistance genes. Therefore, using genetic resources conferring different resistance genes are more advantage in rice improvement. The aim of this research was to survey and screen BB resistance genes in Thai local rice varieties from the National Rice Seed Storage Laboratory for Genetic Resources to gain more genetic diversity for resistance. In this study, the Thai Local rice...
Tipo: PhysicalObject Palavras-chave: Bacterial blight; Molecular marker; BB resistance genes; BB greenhouse screening; โรคขอบใบแห้ง; เครื่องหมายโมเลกุล; ยีนต้านทานโรคขอบใบแห้ง; การคัดเลือก; สภาพเรือนทดลอง; ความต้านทานต่อโรค.
Ano: 2014 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5652
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การใช้ประโยชน์เครื่องหมายโมเลกุลชนิด STS สำหรับงานปรับปรุงพันธุ์ข้าวไทย ให้ต้านทานต่อโรคขอบใบแห้งแบบคงทนถาวร 106
Payorm Cobelli; Somjai Saleeto; Teerada Wangsomboondee; Anuchart Kotchasatit; Jittima Wongnongwa; Varapong Chamarerk; Rasamee Dhitikiattipong; Witchuda Rattanakarn; Nutchakarn Sinprasit; Pornparn Theerattha; Sirada Oncharoen.
Bacterial blight (BB), caused by Xanthomonas oryzae pv. oryzae (Xoo), is one of the most serious diseases of rice in all major rice-growing areas of Thailand. The most effective approach to control BB is the use of resistant varieties which is economically feasible, an environmental-friendly control method and easy for farmers to handle. However, the selection pressure from the usage of resistant cultivars may result in mutation of the pathogen population to overcome the resistance genes. A feasible method to solve this problem is by pyramiding multiple resistance genes, which are board spectrum, into susceptible rice varieties. The aims of this research were to identify the effectiveness of board spectrum BB resistance genes and to better understand the...
Tipo: Collection Palavras-chave: Bacterial blight; Resistance gene; Molecular marker; Board spectrum resistance; Durable resistance; โรคขอบใบแห้ง; ยีนต้านทานโรคขอบใบแห้ง; เครื่องหมายโมเลกุลชนิด STS; ต้านทานแบบกว้างขวาง; ต้านทานแบบคงทนถาวร.
Ano: 2015 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5857
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การใช้ประโยชน์เครื่องหมายโมเลกุลชนิด STS สำหรับงานปรับปรุงพันธุ์ข้าวไทยให้ต้านทานต่อโรคขอบใบแห้งแบบคงทนถาวร 106
Payorm Cobelli; Somjai Saleeto; Teerada Wangsomboondee; Anuchart Kotchasatit; Jittima Wongnongwa; Varapong Chamarerk; Rasamee Dhitikiattipong; Witchuda Rattanakarn; Nutchakarn Sinprasit; Pornparn Theerattha; Sirada Oncharoen.
Bacterial blight (BB), caused by Xanthomonas oryzae pv. oryzae (Xoo), is one of the most serious diseases of rice in all major rice-growing areas of Thailand. The most effective approach to control BB is the use of resistant varieties which is economically feasible, an environmental-friendly control method and easy for farmers to handle. However, the selection pressure from the usage of resistant cultivars may result in mutation of the pathogen population to overcome the resistance genes. A feasible method to solve this problem is by pyramiding the effective and board spectrum of multiple resistance genes into susceptible rice varieties. The aim of this research was to identify the effectiveness and board spectrum of BB resistance genes and better...
Tipo: Collection Palavras-chave: Bacterial blight; Bacterial blight resistance gene; STS molecular marker; Board spectrum resistance; Durable resistance; โรคขอบใบแห้ง; ยีนต้านทานโรคขอบใบแห้ง; เครื่องหมายโมเลกุลชนิด STS; ต้านทานแบบกว้างขวาง; ต้านทานแบบคงทนถาวร.
Ano: 2016 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5950
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การใช้เครื่องหมายโมเลกุลชนิด STSs สำรวจแหล่งพันธุกรรมยีนต้านทาน ต่อโรคขอบใบแห้งของข้าวพันธุ์พื้นเมืองไทยที่เก็บรวบรวมไว้ที่ ศูนย์ปฏิบัติการและเก็บเมล็ดเชื้อพันธุ์ข้าวแห่งชาติ 106
Payorm Cobelli; Jittima Wongnongwa; Varapong Chamarerk; Somsong Chotechuen; Rasamee Dhitikiattipong; Witchuda Rattanakarn; Nuchakarn Sinprasit; Teerada Wangsomboondee.
Bacterial blight (BB) disease of rice, caused by Xanthomonas oryzae pv. oryzae (Xoo), is one of the most serious diseases in all major rice-growing areas of Thailand. The most effective approach to control BB is the use of resistant varieties. However, the selection pressure from the usage of resistant cultivars may result in mutation of the pathogen population to overcome the resistant genes. Therefore, using genetic resources conferring different resistant genes are more advantage in rice improvement. The aim of this research was to survey and screen for BB resistant genes in Thai local rice varieties from the National Rice Seed Storage Laboratory for Genetic Resources to gain more genetic diversity for the resistance. In this study, the Thai Local rice...
Tipo: Collection Palavras-chave: Bacterial Blight (BB); Molecular marker; BB resistance genes; BB greenhouse screening; โรคขอบใบแห้ง; เครื่องหมายโมเลกุล; ยีนต้านทานโรคขอบใบแห้ง; การคัดเลือกความต้านทานต่อโรคขอบใบแห้งในสภาพเรือนทดลอง.
Ano: 2014 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5935
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การตรวจวิเคราะห์ข้าวและสินค้าข้าวดัดแปรพันธุกรรมด้วยปฏิกิริยาลูกโซ่ ในสภาพเวลาจริง 106
Varapong Chamarerk; Roongnapa Kawichai; Payorm Cobelli; Poonsak Mekwatanakarn.
Genetically modified (GM) insecticidal rice line, Bt63 (Shanyou63), has been developed in China and found unauthorized in processed rice products in many countries. This transgenic rice contains a copy of the synthetic DNA sequence with two genes: CryIA(b) and CryIA(c). These genes encode for the respective Bt toxins which is lethal to Lepidoptera. The aim of this study was to determine the contamination of Bt63 in non-labelled commercially available processed rice products from supermarkets. In this study, a real-time Polymerase Chain Reaction (PCR) was used to simultaneously determine the contamination of Bt63. The survey was conducted on a total of 21 and 42 non-labeled samples of processed rice products collected in the years 2011 and 2012,...
Tipo: Collection Palavras-chave: GM rice; Bt63; Real-time PCR; ข้าวดัดแปรพันธุกรรม; Bt63; ปฏิกิริยาลูกโซ่โพลีเมอเรสแบบเวลาจริง.
Ano: 2015 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5923
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