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	Provedor de dados BJMBR (1) Autor Bosetto,M.C. (1) Castro,L.I.R. de (1) Cicarelli,R.M.B. (1) Peixoto,M.S. (1) Palavra-chave Autoantibodies (1) Chagas' disease (1) Cross-reactivity (1) HeLa cells (1) Small nuclear ribonucleoprotein (1) Trypanosoma cruzi (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Ano 2004 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Presence of autoantibodies against HeLa small nuclear ribonucleoproteins in chagasic and non-chagasic cardiac patients BJMBR Bosetto,M.C.; Peixoto,M.S.; Castro,L.I.R. de; Cicarelli,R.M.B.. We detected anti-human small nuclear ribonucleoprotein (snRNP) autoantibodies in chagasic patients by different immunological methods using HeLa snRNPs. ELISA with Trypanosoma cruzi total lysate antigen or HeLa human U small nuclear ribonucleoproteins (UsnRNPs) followed by incubation with sera from chronic chagasic and non-chagasic cardiac patients was used to screen and compare serum reactivity. Western blot analysis using a T. cruzi total cell extract was also performed in order to select some sera for Western blot and immunoprecipitation assays with HeLa nuclear extract. ELISA showed that 73 and 95% of chronic chagasic sera reacted with HeLa UsnRNPs and T. cruzi antigens, respectively. The Western blot assay demonstrated that non-chagasic cardiac sera... Tipo: Info:eu-repo/semantics/article Palavras-chave: Chagas' disease; Autoantibodies; Small nuclear ribonucleoprotein; Trypanosoma cruzi; HeLa cells; Cross-reactivity. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000100006 Registros recuperados: 1 Primeira ... 1 ... Última

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