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Comparison of multiplex-PCR and antigen detection for differential diagnosis of Entamoeba histolytica BJID
Santos,Helena Lúcia Carneiro; Peralta,Regina Helena Saramago; Macedo,Heloisa Werneck de; Barreto,Magali Gonçalves Muniz; Peralta,José Mauro.
Amebiasis is an infection caused by Entamoeba histolytica. However, differentiation between E. histolytica and Entamoeba dispar, which are morphologically identical species, is essential for treatment decision, precaution of the invasive disease and public health. The purpose of the present study was to evaluate a Multiplex -PCR for detection and differentiation of E. histolytica from E. dispar from fresh stool samples in comparison with the coproantigen commercial ELISA. Microscopic examination of stools using the Coprotest method, detection of stool antigen by enzyme-linked immunosorbent assay kit and a home made Multiplex-PCR, were used for the diagnosis of amoebiasis infection. Analysis of the 127 stools samples by microscopy examination demonstrated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Multiplex-PCR; Entamoeba histolytica; Amebiasis.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000300013
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Genital schistosomiasis mansoni concomitant to genital tumor in areas of low endemicity: challenging diagnosis BJID
Cavalcanti,Marta G; Gonçalves,Margareth MI; Barreto,Magali M; Silva,Aline Helen da; Madi,Kalil; Peralta,José Mauro; Igreja,Ricardo P.
Genital infection by Schistosoma mansoni is usually misdiagnosed in individuals who reside in, or travel to endemic areas. We describe two cases of genital tumor associated with S. mansoni infection manifested by methrorragy. Surgical specimens revealed leiomyomas in both cases associated with S. mansoni. In one of them, granulomas were found in the ovary and in the other they were found in the uterine tube. Although none presented intestinal/hepatic disease, fecal egg excretion was detected in one. Both had elevated pretreatment antibody reactivity to S. mansoni antigen, but follow-up showed different outcomes. Schistosomiasis should be considered as a diagnosis in individuals with methrorragy residing in or having traveled to endemic areas. Since...
Tipo: Info:eu-repo/semantics/article Palavras-chave: S. mansoni; Female genital infection; Serology; Low endemic area.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000200016
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