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Patterns of intracellular cytokines in CD4 and CD8 T cells from patients with mycobacterial infections BJMBR
Antas,P.R.Z.; Sales,J.S.; Pereira,K.C.; Oliveira,E.B.; Cunha,K.S.; Sarno,E.N.; Sampaio,E.P..
Using a short-term bulk culture protocol designed for an intracellular-staining method based on a flow cytometry approach to the frequencies of cytokine-producing cells from tuberculosis and leprosy patients, we found distinct patterns of T cell subset expression. The method also reveals the profile of peak cytokine production and can provide simultaneous information about the phenotype of cytokine-producing cells, providing a reliable assay for monitoring the immunity of these patients. The immune response of Mycobacterium leprae and purified protein derivative (PPD) in vitro to a panel of mycobacteria-infected patients from an endemic area was assessed in primary mononuclear cell cultures. The kinetics and source of the cytokine pattern were measured at...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tuberculosis; Leprosy; IFN-gamma; TNF-alpha; Anti-hCD28 mAb.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000800003
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