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Genetic variability of two populations of Pseudoplatystoma reticulatum from the Upper Paraguay River Basin Genet. Mol. Biol.
Abreu,Marcia Matos de; Pereira,Luiz Henrique Garcia; Vila,Vilma Barretto; Foresti,Fausto; Oliveira,Claudio.
Catfishes of the genus Pseudoplatystoma are very important species due to both their high commercial value and their ecological role as voracious predators. They undertake lengthy migratory movements during their life-cycle, this including reproductive migration which occurs from October to December in the rainy season. In the present study, seven microsatellite loci were analyzed to access genetic variability in two samples of P. reticulatum from the Upper Paraguay Basin. The loci were highly polymorphic (mean = 7.28). According to all analysis, the two samples of P. reticulatum revealed pronounced genetic differentiation. Fst value was 0.2290, Rst value 0.1067 and AMOVA 22.90% (Fst) and 10.67% (Rst), all being highly significant (p < 0.001). The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fish; Population genetics; Microsatellite; Homing; Population structure.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400032
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DNA barcode of Parodontidae species from the La Plata river basin - applying new data to clarify taxonomic problems Neotropical Ichthyology
Bellafronte,Elisangela; Mariguela,Tatiane Casagrande; Pereira,Luiz Henrique Garcia; Oliveira,Claudio; Moreira-Filho,Orlando.
In the past years, DNA barcoding has emerged as a quick, accurate and efficient tool to identify species. Considering the difficulty in identifying some Parodontidae species from the La Plata basin and the absence of molecular data for the group, we aimed to test the effectiveness of DNA barcoding and discuss the importance of using different approaches to solve taxonomic problems. Eight species were analyzed with partial sequences of Cytochrome c oxidase I. The mean intraspecific K2P genetic distance was 0.04% compared to 4.2% for mean interspecific K2P genetic distance. The analyses of distance showed two pairs of species with K2P genetic divergence lower than 2%, but enough to separate these species. Apareiodon sp. and A. ibitiensis, considered as the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Apareiodon; COI gene; Cytogenetics; Fish identification; Morphology.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000300497
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Cytogenetic and molecular markers reveal the complexity of the genus Piabina Reinhardt, 1867 (Characiformes: Characidae) Neotropical Ichthyology
Pazian,Marlon Felix; Pereira,Luiz Henrique Garcia; Shimabukuru-Dias,Cristiane Kioko; Oliveira,Claudio; Foresti,Fausto.
Cytogenetic and molecular analyses were carried out in fish representative of the genus Piabina. This study specifically involved the species P. argentea and P. anhembi collected from areas of the Paranapanema and Tietê River basins, Brazil. Our findings suggest that fish classified as Piabina argentea in the Paranapanema and Tietê Rivers may represent more than one species. The samples analyzed differed by cytogenetic particularities and molecular analyses using partial sequences of the genes COI and CytB as genetic markers revealed three distinct groups of P. argentea with genetic distances sufficient to support the conclusion that the three samples analyzed are three distinct taxonomic units.
Tipo: Info:eu-repo/semantics/article Palavras-chave: DNA Barcoding; Freshwater fishes; Fish; MtDNA; 18S rDNA; 5S rDNA.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252012000200008
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