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Oliveira,A.L.; Pereira,M.T.; Bueno,P.H.S.; Oliveira,R.B.P.; Pinto,F.C.; Correia,R.F.; Machado,M.M.. |
Foram avaliados os efeitos da radiação gama nas doses de 1,5 e 3,0kGy, em peitos de frango embalados em embalagem convencional e a vácuo. As amostras embaladas foram submetidas à irradiação e estocadas sob refrigeração (4ºC) por 30 dias. A radiação mostrou-se eficiente na redução de mesófilos aeróbios estritos e facultativos viáveis, coliformes totais e termotolerantes, com destaque para Salmonella spp. Os bolores e leveduras mostraram-se bastante resistentes à radiação. Os resultados sugerem que a dose de 3,0kGy pode ser utilizada no controle da microbiota do peito de frango armazenado sob refrigeração, sendo uma alternativa para o aumento da vida de prateleira por até 10 dias, ao garantir a segurança do consumidor pela eliminação de possíveis patógenos. |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Peito de frango; Radiação gama; Irradiação; Microbiologia; Vida de prateleira. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352009000500026 |
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Pereira,M.T.; Silva-Alves,J.M.; Martins-José,A.; Lopes,J.C.D.; Santoro,M.M.. |
Serine-proteases are involved in vital processes in virtually all species. They are important targets for researchers studying the relationships between protein structure and activity, for the rational design of new pharmaceuticals. Trypsin was used as a model to assess a possible differential contribution of hydration water to the binding of two synthetic inhibitors. Thermodynamic parameters for the association of bovine ß-trypsin (homogeneous material, observed 23,294.4 ± 0.2 Da, theoretical 23,292.5 Da) with the inhibitors benzamidine and berenil at pH 8.0, 25ºC and with 25 mM CaCl2, were determined using isothermal titration calorimetry and the osmotic stress method. The association constant for berenil was about 12 times higher compared to the one for... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Benzamidine; Berenil; Calorimetry; Protein modeling; Trypsin; Osmotic stress. |
Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001100005 |
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Dolabela,M.F.; Pereira,M.T.; Salas,C.E.; Steffani,G.M.; Nelson,D.L.; Piló-Veloso,D.; Lopes,M.T.P.. |
We examined the radioprotective effect of aminothiol 2-N-propylamine-cyclo-hexanethiol (20-PRA) on a human leukemic cell line (K562) following various radiation doses (5, 7.5 and 20 Gy) using a source of 60Co <FONT FACE="Symbol">g</FONT>-rays. At 5 Gy and 1 nM 20-PRA, a substantial protective effect (58%) was seen 24 h after irradiation, followed by a decrease at 48 h (11%). At the high radiation dose (20 Gy) a low protective effect was also seen (35%). In addition, the antitumorigenic potential of 10 nM 20-PRA was shown by the inhibition of crown gall formation induced by Agrobacterium tumefaciens. The radioprotective potency of 20-PRA is 105-106 times higher than that of the aminothiol WR-1065 (N-(2-mercaptoethyl)-1,3-diaminopropane) whose... |
Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Aminothiols; Cytotoxic effect; Radioprotector; Antitumorigenic effect. |
Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000800010 |
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