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| Valadares,Rafael Borges da Silva; Otero,Joel Tupac; Pereira,Marlon Corrêa; Cardoso,Elke Jurandy Bran Nogueira. |
| In Orchidaceae, association with symbiotic fungi is required for seed germination and seedling development, thereby being the main energy source during the first steps of germination. Colombia is one of the countries with the greatest biodiversity of orchids, with an estimated 3,200 species, but few studies on orchid mycorrhiza have been conducted. In our study, we isolated and sequenced the internal transcribed spacer rDNA region of fungi from two co-occurring Colombian epiphytic orchids, I. utricularioides and P. pusilla, both belonging to the subtribe Oncidiinae. All sequences were recognized as belonging to the genus Ceratobasidium, known to be a common orchid mycorrhizal fungus in both tropical and temperate orchids. One sequence was 100% similar to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Co-existence; ITS phylogeny; Mycorrhiza; Orchidaceae; Specificity. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062015000100040 |
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| Guimarães,Fernanda Aparecida Rodrigues; Pereira,Marlon Corrêa; Felício,Cristiane da Silva; Torres,Denise Pereira; Oliveira,Sabrina Feliciano; Veloso,Tomás Gomes Reis; Kasuya,Maria Catarina Megumi. |
| In nature, orchid seeds obtain the nutrients necessary for germination by degrading intracellular fungal structures formed after colonization of the embryo by mycorrhizal fungi. Protocols for asymbiotic germination of orchid seeds typically use media with high concentrations of soluble carbohydrate and minerals. However, when reintroduced into the field, seedlings obtained via asymbiotic germination have lower survival rates than do seedlings obtained via symbiotic germination. Tree fern fiber, the ideal substrate for orchid seedling acclimatization, is increasingly scarce. Here, we evaluated seed germination and protocorm development of Cyrtopodium glutiniferum Raddi cultivated in asymbiotic media (Knudson C and Murashige & Skoog) and in oatmeal... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Epulorhiza sp.; Symbiosis; Orchid; Substrate; Fern fiber. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062013000300016 |
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| Pereira,Marlon Corrêa; Rocha,Diego Ismael; Veloso,Tomás Gomes Reis; Pereira,Olinto Liparini; Francino,Dayana Maria Teodoro; Meira,Renata Maria Strozi Alves; Kasuya,Maria Catarina Megumi. |
| Cyrtopodium glutiniferum is an endemic orchid of Brazil with potential medicinal and ornamental applications. As mycorrhizal fungi are essential for the initiation of the orchid life cycle, the aim of this study was to determine the strains of mycorrhizal fungi suitable for seed germination and protocorm development of C. glutiniferum and to characterize the symbiotic development of protocorms. Seeds of C. glutiniferum were inoculated with nine mycorrhizal fungi, Epulorhiza spp., Ceratorhiza spp., Rhizoctonia sp., originally isolated from Brazilian neotropical orchids. Only Epulorhiza isolates promoted seed germination and protocorm development. Three Epulorhiza isolates (M1, M6 = E. epiphytica, M20 = Epulorhiza sp.) promoted protocorm development until... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ceratorhiza; Epulorhiza; Orchid conservation; Seedling production; Symbiosis. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062015000400567 |
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| Pereira,Marlon Corrêa; Vieira,Nívea Moreira; Tótola,Marcos Rogério; Kasuya,Maria Catarina Megumi. |
| Rhizoctonia-like fungi are the main mycorrhizal fungi in orchid roots. Morphological characterization and analysis of conserved sequences of genomic DNA are frequently employed in the identification and study of fungi diversity. However, phytopathogenic Rhizoctonia-like fungi have been reliably and accurately characterized and identified through the examination of the fatty acid composition. To evaluate the efficacy of fatty acid composition in characterizing and identifying Rhizoctonia-like mycorrhizal fungi in orchids, three Epulorhiza spp. mycorrhizal fungi from Epidendrum secundum, two unidentified fungi isolated from Epidendrum denticulatum, and a phytopathogenic fungus, Ceratorhiza sp. AGC, were grouped based on the profile of their fatty acids,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rhizoctonia-like fungi; Epidendrum spp. orchids; FAME; MIDI method; ITS sequence analysis; Fungos rizoctonioides; Orquídeas Epidendrum spp; FAME; Método MIDI; Análise da sequência da região ITS. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832011000400009 |
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