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Isolation and characterization of mesenchymal stem/stromal cells from Ctenomys minutus Genet. Mol. Biol.
Pereira,Mayra Ramos de Jesus; Pinhatti,Valéria Rodrigues; Silveira,Maiele Dornelles da; Matzenbacher,Cristina Araujo; Freitas,Thales Renato Ochotorena de; Silva,Juliana da; Camassola,Melissa; Nardi,Nance Beyer.
Abstract Mesenchymal stem/stromal cells (MSCs) are multipotent cells distributed in all tissues and characterized by adherence, morphology, immunophenotype and trilineage differentiation potential. The present study aimed to isolate and characterize adherent MSC-like populations from different tissues of Ctenomys minutus, a threatened wildlife rodent popularly known as tuco-tuco. Adherent cells were isolated from bone marrow, brain, liver, pancreas and adipose tissue of three adult animals collect in southern Brazil. Cultures showed typical morphology and proliferation potential. Adipose-derived MSCs showed trilineage potential. Cultures derived from adipose tissue, bone marrow and brain were immunophenotyped with negative results for CD31, CD44, CD45,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mesenchymal stem/stromal cells; Tuco-tuco; Ctenomys minutus.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000500870
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