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Desvio para a esquerda associado a compartimento medular de reserva dos neutrófilos não esgotado em cadelas portadoras de piometra Arq. Bras. Med. Vet. Zootec.
Paes,P.R.O.; Andrade,M.G.M.G.; Leme,F.O.P.; Veloso,L.B.; Pereira,R.D.O.; Malm,C.; Melo,M.M.; Silva,N.A.M..
From fifteen female dogs with clinical diagnoses of pyometra, hematological exams were performed at three times: M0 (prior to the surgery), M24 (24h after ovarysalpingohisterectomy-OSH) and M48 (48h after OSH). Anemia was seen in 80% of the cases, characterized as mild normocytic normochromic type. The means of total leukocyte counts were 27.043, 57.940 and 40.139 céls/µL in M0, M24 and M48. A total of80% of the animals presented neutrophilic left shift in all moments. During medullar exams, the cellular, iron reserve and megakaryocytic concentration were raised as well as the ME ratio, showing a value of 26,3:1,0, probably due to the elevation of granular proliferation and maturation compartment, as the mean of the reserve compartment was within normal...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Piometra; Hematologia; Medula óssea; Desvio para a esquerda; Mielograma.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000501611
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Behavior of mesenchymal stem cells stained with 4&', 6-diamidino-2-phenylindole dihydrochloride (DAPI) in osteogenic and non osteogenic cultures Biocell
Ocarino,N.M.; Bozzi,A.; Pereira,R.D.O.; Breyner,N.M.; Silva,V.L.; Castanheira,P.; Goes,A.M.; Serakides,R..
4&', 6-diamidino-2-phenylindole dihydrochloride (DAPI) is a DNA dye widely used to mark and trace stem cells in therapy. We here studied the effect of DAPI staining on the behavior of mesenchymal stem cells cultured in either a control, non-osteogenic medium or in an osteogenic differentiation medium. In the control medium, the number of stem cells/field, as well as the number of fluorescent cells/field increased up to the sixth day in both control and DAPI-treated cultures. Afterwards, both the number of fluorescent cells and their fluorescence intensity decreased. Control cells were fusiform and with some long extensions that apparently linked them to neighboring cells, while DAPI-treated cells were mostly round cells with fine and short extensions....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bone marrow; Cell cultures; Rat; Osteogenic differentiation.
Ano: 2008 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452008000200003
Registros recuperados: 2
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